Detailed information of TA system TA181583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 242448..242989
• Replicon: chromosome
• Accession: NZ_CP064682
• Organism: Escherichia coli K-12 strain BZKR2G40

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q47149
• Locus tag: IVK99_RS01130
• Protein ID: WP_000615983.1
• Coordinates: 242448..242726 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q47150
• Locus tag: IVK99_RS01135
• Protein ID: WP_000729703.1
• Coordinates: 242729..242989 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVK99_RS01115	240030..240608	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
IVK99_RS01120	240814..241581	+	768	WP_000333380.1	class II glutamine amidotransferase	-
IVK99_RS01125	241552..242292	-	741	WP_001225679.1	murein L,D-transpeptidase	-
IVK99_RS01130	242448..242726	-	279	WP_000615983.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
IVK99_RS01135	242729..242989	-	261	WP_000729703.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
IVK99_RS01140	243199..243948	+	750	WP_000056849.1	C40 family peptidase	-
IVK99_RS01145	244124..244621	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
IVK99_RS01150	244845..246557	-	1713	Protein_222	flagellar biosynthesis protein FlhA	-
IVK99_RS01155	246529..247314	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T181583 WP_000615983.1 NZ_CP064682:c242726-242448 [Escherichia coli K-12]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T181583 NZ_CP085976:c2196007-2195717 [Blautia massiliensis]
ATGAGGAAAACAAAGTACACCGTCAAGTACACGACCGCTTTCAAAAAGGACTACAAGCGGGCAATCAAGCGCGGCCTGAA
AATCGAATTGCTGGAACAGGTCGTGGCGCTGCTGTCGATGGGCGAACCGCTGCCGGATAAAAACCGCGATCACGATTTGT
CCGGCGATTGGGCAGGCCATCGAGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT181583 WP_000729703.1 NZ_CP064682:c242989-242729 [Escherichia coli K-12]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT181583 NZ_CP085976:c2196282-2196004 [Blautia massiliensis]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAGCT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAGGAAACGATTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGATGC
AATGACCTTGACGAGTTGTTTGCCGACCTGAAAAAATGA

Structures

Toxin

• PDB: 4Q2U

Antitoxin

• AlphaFold DB: A0A5F1F716