Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 248492..249186 Replicon chromosome
Accession NZ_CP064681
Organism Escherichia coli K-12 strain BZKR3G40

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag IVL00_RS01170 Protein ID WP_001263489.1
Coordinates 248788..249186 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag IVL00_RS01165 Protein ID WP_000554758.1
Coordinates 248492..248785 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL00_RS01145 244124..244621 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IVL00_RS01150 244845..246557 - 1713 Protein_222 flagellar biosynthesis protein FlhA -
IVL00_RS01155 246529..247314 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
IVL00_RS01160 247385..248440 + 1056 WP_001226164.1 DNA polymerase IV -
IVL00_RS01165 248492..248785 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IVL00_RS01170 248788..249186 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IVL00_RS01175 249196..249648 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
IVL00_RS01180 249966..250172 + 207 Protein_228 RtcB family protein -
IVL00_RS01185 250168..250689 + 522 Protein_229 peptide chain release factor H -
IVL00_RS01190 250746..252203 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
IVL00_RS01195 252464..252922 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 253518..253598 + 81 NuclAT_12 - -
- 253518..253598 + 81 NuclAT_12 - -
- 253518..253598 + 81 NuclAT_12 - -
- 253518..253598 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T181541 WP_001263489.1 NZ_CP064681:248788-249186 [Escherichia coli K-12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T181541 NZ_CP085951:1144842-1145105 [Haemophilus influenzae]
ATGATTTCAGTGAGTGAGGAATATAAAAGAGAATTTAAAAAACATATTCCTCTTATTATAAATAGTCCAGAATATATTGA
AGTTATGTATTGTCTTTTGAATAGCTTACCATTGCCAGAAAAATATAAAGATCACGCTTTAACTGGCAATTGGAAAGGTT
GGCGAGATTGCCATATTAAGAATGATCTCGTGCTTATTTATAAAATTGTGGGGGATGAAATAAGACTAGCTCGTCTTAAT
ACCCATCCAGAAATTTTTGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT181541 WP_000554758.1 NZ_CP064681:248492-248785 [Escherichia coli K-12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT181541 NZ_CP085951:1144570-1144845 [Haemophilus influenzae]
ATGGCTGCAGTTAGCTTTCGAGTACCTGATGAAACTAAAAATATAGCGTTTGAAGTGATTAAAAGTTATGGTCTTTCCCC
AACTCAAGCACTTAATATGTTTTTAGCTCAAATAGCTAAAACGAAAGCGATTCCAGTATCATTAGATTATCAAACTGAAC
AGATGCCTAACTCAAAAACGATAATGGCTATGGCAGAGTTGGATAATCATAATGGGCGTGAATACAAAACATTGGAGGAA
TTCCATCAAGCGATGCTATCGGAGATGAAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References