Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2082690..2083192 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IVL01_RS10315 Protein ID WP_000767829.1
Coordinates 2082690..2082944 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IVL01_RS10320 Protein ID WP_001259255.1
Coordinates 2082941..2083192 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS10285 2077705..2077932 - 228 WP_000234896.1 sulfurtransferase TusA -
IVL01_RS10290 2077946..2079004 - 1059 WP_000492339.1 transport protein YeeE -
IVL01_RS10295 2079183..2080541 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IVL01_RS10300 2080531..2080593 - 63 WP_010723108.1 membrane protein YoeI -
IVL01_RS10305 2080808..2081737 - 930 WP_000803351.1 LysR family transcriptional regulator -
IVL01_RS10310 2081783..2082607 - 825 WP_000754737.1 SDR family oxidoreductase -
IVL01_RS10315 2082690..2082944 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IVL01_RS10320 2082941..2083192 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IVL01_RS10325 2083475..2083525 + 51 WP_001364200.1 his operon leader peptide -
IVL01_RS10330 2083671..2084570 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IVL01_RS10335 2084576..2085880 + 1305 WP_000009594.1 histidinol dehydrogenase -
IVL01_RS10340 2085877..2086947 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
IVL01_RS10345 2086947..2088014 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T181523 WP_000767829.1 NZ_CP064680:c2082944-2082690 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T181523 NZ_CP085945:c3138001-3137729 [Mycobacterium intracellulare]
ATGCAACCGGCGGATTACACGGTTCGGTTCACCGCCACCGCGCGGCGTGACCTGCACAAGCTGCCACCGCGCGTGCTTTC
GGCCGTGATTGAGTTCGCATTCGGCGACCTGGCGCACGAACCACGCCGCGTCGGCAAGCCGCTGCGGCGTGAACTCGCCG
GGCTGTATAGCGCCCGGCGCGGTCCGTACCGCCTCTTGTACCGGATCGACGGGGGCCGCTCACGGGTGCTGATTCAGCGT
GTCGATCATCGCGCAGATGTTTACAGGCGTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT181523 WP_001259255.1 NZ_CP064680:c2083192-2082941 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT181523 NZ_CP085945:c3138282-3138001 [Mycobacterium intracellulare]
GTGCGAATACTTCCCATTTCGAAGATGAAGGACAAGCTCAACGAGTACGTCGACGCGGTCGCCAAAACGCAGGATCAGAT
CACCATCACCAAGAACGGTGCGCCTGCGGCGGTGCTCGTCGGCGCCGAGGAGTGGGAATCACTCCAGGAGACGCTGTACT
GGCTTTCCAGGCCCGGGATCAAGGATTCGATCGCCGAGGCCGACGCCGACATTGCCGCTGGGCGCACTTACGACGAGGAC
GAAATTCGCGCCGAGTTCGGCGTGCCTCGGCGCGCCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References