181523

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2082690..2083192 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IVL01_RS10315 Protein ID WP_000767829.1
Coordinates 2082690..2082944 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IVL01_RS10320 Protein ID WP_001259255.1
Coordinates 2082941..2083192 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS10285 2077705..2077932 - 228 WP_000234896.1 sulfurtransferase TusA -
IVL01_RS10290 2077946..2079004 - 1059 WP_000492339.1 transport protein YeeE -
IVL01_RS10295 2079183..2080541 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IVL01_RS10300 2080531..2080593 - 63 WP_010723108.1 membrane protein YoeI -
IVL01_RS10305 2080808..2081737 - 930 WP_000803351.1 LysR family transcriptional regulator -
IVL01_RS10310 2081783..2082607 - 825 WP_000754737.1 SDR family oxidoreductase -
IVL01_RS10315 2082690..2082944 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IVL01_RS10320 2082941..2083192 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IVL01_RS10325 2083475..2083525 + 51 WP_001364200.1 his operon leader peptide -
IVL01_RS10330 2083671..2084570 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IVL01_RS10335 2084576..2085880 + 1305 WP_000009594.1 histidinol dehydrogenase -
IVL01_RS10340 2085877..2086947 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
IVL01_RS10345 2086947..2088014 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T181523 WP_000767829.1 NZ_CP064680:c2082944-2082690 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T181523 NZ_CP085945:c3138001-3137729 [Mycobacterium intracellulare]
ATGCAACCGGCGGATTACACGGTTCGGTTCACCGCCACCGCGCGGCGTGACCTGCACAAGCTGCCACCGCGCGTGCTTTC
GGCCGTGATTGAGTTCGCATTCGGCGACCTGGCGCACGAACCACGCCGCGTCGGCAAGCCGCTGCGGCGTGAACTCGCCG
GGCTGTATAGCGCCCGGCGCGGTCCGTACCGCCTCTTGTACCGGATCGACGGGGGCCGCTCACGGGTGCTGATTCAGCGT
GTCGATCATCGCGCAGATGTTTACAGGCGTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT181523 WP_001259255.1 NZ_CP064680:c2083192-2082941 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT181523 NZ_CP085945:c3138282-3138001 [Mycobacterium intracellulare]
GTGCGAATACTTCCCATTTCGAAGATGAAGGACAAGCTCAACGAGTACGTCGACGCGGTCGCCAAAACGCAGGATCAGAT
CACCATCACCAAGAACGGTGCGCCTGCGGCGGTGCTCGTCGGCGCCGAGGAGTGGGAATCACTCCAGGAGACGCTGTACT
GGCTTTCCAGGCCCGGGATCAAGGATTCGATCGCCGAGGCCGACGCCGACATTGCCGCTGGGCGCACTTACGACGAGGAC
GAAATTCGCGCCGAGTTCGGCGTGCCTCGGCGCGCCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References