181480

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2082692..2083194 Replicon chromosome
Accession NZ_CP064679
Organism Escherichia coli K-12 strain CETR2G40

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IVL02_RS10310 Protein ID WP_000767829.1
Coordinates 2082692..2082946 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IVL02_RS10315 Protein ID WP_001259255.1
Coordinates 2082943..2083194 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL02_RS10280 2077707..2077934 - 228 WP_000234896.1 sulfurtransferase TusA -
IVL02_RS10285 2077948..2079006 - 1059 WP_000492339.1 transport protein YeeE -
IVL02_RS10290 2079185..2080543 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IVL02_RS10295 2080533..2080595 - 63 WP_010723108.1 membrane protein YoeI -
IVL02_RS10300 2080810..2081739 - 930 WP_000803351.1 LysR family transcriptional regulator -
IVL02_RS10305 2081785..2082609 - 825 WP_000754737.1 SDR family oxidoreductase -
IVL02_RS10310 2082692..2082946 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IVL02_RS10315 2082943..2083194 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IVL02_RS10320 2083477..2083527 + 51 WP_001364200.1 his operon leader peptide -
IVL02_RS10325 2083673..2084572 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IVL02_RS10330 2084578..2085882 + 1305 WP_000009594.1 histidinol dehydrogenase -
IVL02_RS10335 2085879..2086949 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
IVL02_RS10340 2086949..2088016 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T181480 WP_000767829.1 NZ_CP064679:c2082946-2082692 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T181480 NZ_CP064679:c2082946-2082692 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT181480 WP_001259255.1 NZ_CP064679:c2083194-2082943 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT181480 NZ_CP064679:c2083194-2082943 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References