Detailed information of TA system TA181266

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 32267..32472
• Replicon: plasmid unnamed_3
• Accession: NZ_CP064427
• Organism: Enterococcus faecium strain PR01996-12

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: IUH71_RS15095
• Protein ID: WP_002295624.1
• Coordinates: 32267..32389 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	32371..32472 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUH71_RS15055 (27343)	27343..27546	-	204	WP_002300097.1	hypothetical protein	-
IUH71_RS15060 (27706)	27706..28281	-	576	WP_002300096.1	recombinase family protein	-
IUH71_RS15065 (28297)	28297..28902	-	606	WP_002300095.1	Fic family protein	-
IUH71_RS15070 (29508)	29508..29687	-	180	WP_002305204.1	hypothetical protein	-
IUH71_RS15850 (29703)	29703..29903	-	201	WP_010729220.1	hypothetical protein	-
IUH71_RS15075 (30068)	30068..30775	-	708	WP_002305202.1	hypothetical protein	-
IUH71_RS15080 (30768)	30768..31205	-	438	WP_002287515.1	hypothetical protein	-
IUH71_RS15085 (31210)	31210..31410	-	201	WP_002305199.1	hypothetical protein	-
IUH71_RS15090 (31853)	31853..32113	-	261	WP_049055261.1	hypothetical protein	-
IUH71_RS15095 (32267)	32267..32389	+	123	WP_002295624.1	putative holin-like toxin	Toxin
- (32371)	32371..32472	-	102	NuclAT_0	-	Antitoxin
- (32371)	32371..32472	-	102	NuclAT_0	-	Antitoxin
- (32371)	32371..32472	-	102	NuclAT_0	-	Antitoxin
IUH71_RS15100 (32549)	32549..32806	-	258	WP_002305195.1	hypothetical protein	-
IUH71_RS15105 (32828)	32828..33313	-	486	WP_002295626.1	single-stranded DNA-binding protein	-
IUH71_RS15110 (33316)	33316..34215	-	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
IUH71_RS15115 (34231)	34231..34824	-	594	WP_002287520.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
IUH71_RS15120 (34898)	34898..35047	-	150	WP_002295632.1	hypothetical protein	-
IUH71_RS15125 (35063)	35063..37267	-	2205	WP_025481008.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T181266 WP_002295624.1 NZ_CP064427:32267-32389 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T181266 NZ_CP085816:2979997-2980215 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Nucleotide

• Length: 102 bp

>AT181266 NZ_CP064427:c32472-32371 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin