Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3498620..3499290 Replicon chromosome
Accession NZ_CP064405
Organism Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag IRY56_RS16290 Protein ID WP_003899954.1
Coordinates 3498620..3498964 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag IRY56_RS16295 Protein ID WP_003899955.1
Coordinates 3498961..3499290 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRY56_RS16260 3493693..3494553 + 861 WP_003416628.1 alpha/beta hydrolase -
IRY56_RS16265 3494528..3495043 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
IRY56_RS16270 3495283..3495576 + 294 WP_003416635.1 hypothetical protein -
IRY56_RS16275 3495864..3497153 + 1290 WP_003416640.1 ATP-binding protein -
IRY56_RS16280 3497500..3497934 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
IRY56_RS16285 3497937..3498389 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IRY56_RS16290 3498620..3498964 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IRY56_RS16295 3498961..3499290 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
IRY56_RS16300 3499828..3500175 + 348 WP_003899956.1 DUF2384 domain-containing protein -
IRY56_RS16305 3500172..3500792 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
IRY56_RS16310 3500952..3502217 - 1266 WP_003909837.1 hypothetical protein -
IRY56_RS16315 3502385..3502594 + 210 WP_003416778.1 hypothetical protein -
IRY56_RS16320 3502841..3503875 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T181257 WP_003899954.1 NZ_CP064405:3498620-3498964 [Mycobacterium tuberculosis variant bovis BCG]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T181257 NZ_CP085815:3873342-3873617 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT181257 WP_003899955.1 NZ_CP064405:3498961-3499290 [Mycobacterium tuberculosis variant bovis BCG]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT181257 NZ_CP085815:3873079-3873339 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References