Detailed information of TA system TA181257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3498620..3499290
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: IRY56_RS16290
• Protein ID: WP_003899954.1
• Coordinates: 3498620..3498964 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: IRY56_RS16295
• Protein ID: WP_003899955.1
• Coordinates: 3498961..3499290 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS16260	3493693..3494553	+	861	WP_003416628.1	alpha/beta hydrolase	-
IRY56_RS16265	3494528..3495043	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
IRY56_RS16270	3495283..3495576	+	294	WP_003416635.1	hypothetical protein	-
IRY56_RS16275	3495864..3497153	+	1290	WP_003416640.1	ATP-binding protein	-
IRY56_RS16280	3497500..3497934	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
IRY56_RS16285	3497937..3498389	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IRY56_RS16290	3498620..3498964	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IRY56_RS16295	3498961..3499290	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
IRY56_RS16300	3499828..3500175	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
IRY56_RS16305	3500172..3500792	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
IRY56_RS16310	3500952..3502217	-	1266	WP_003909837.1	hypothetical protein	-
IRY56_RS16315	3502385..3502594	+	210	WP_003416778.1	hypothetical protein	-
IRY56_RS16320	3502841..3503875	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T181257 WP_003899954.1 NZ_CP064405:3498620-3498964 [Mycobacterium tuberculosis variant bovis BCG]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T181257 NZ_CP085815:3873342-3873617 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT181257 WP_003899955.1 NZ_CP064405:3498961-3499290 [Mycobacterium tuberculosis variant bovis BCG]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT181257 NZ_CP085815:3873079-3873339 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6