Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2238533..2239452 Replicon chromosome
Accession NZ_CP064405
Organism Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag IRY56_RS10340 Protein ID WP_003900449.1
Coordinates 2238847..2239452 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag IRY56_RS10335 Protein ID WP_003410124.1
Coordinates 2238533..2238838 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRY56_RS10305 2233544..2234800 - 1257 WP_003410095.1 HNH endonuclease -
IRY56_RS10310 2235154..2235729 + 576 WP_003410100.1 hypothetical protein -
IRY56_RS10315 2235726..2236766 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
IRY56_RS10320 2237008..2237727 + 720 WP_010950643.1 DUF433 domain-containing protein -
IRY56_RS10325 2237717..2238133 + 417 WP_003410114.1 hypothetical protein -
IRY56_RS10330 2238149..2238448 - 300 WP_003410120.1 hypothetical protein -
IRY56_RS10335 2238533..2238838 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
IRY56_RS10340 2238847..2239452 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IRY56_RS10345 2239477..2239836 - 360 WP_003410131.1 hypothetical protein -
IRY56_RS10350 2239996..2240673 - 678 WP_003900450.1 hypothetical protein -
IRY56_RS10355 2240625..2240861 - 237 WP_003410133.1 hypothetical protein -
IRY56_RS10360 2241068..2241964 - 897 WP_011799238.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T181240 WP_003900449.1 NZ_CP064405:c2239452-2238847 [Mycobacterium tuberculosis variant bovis BCG]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T181240 NZ_CP085814:c1738827-1738609 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT181240 WP_003410124.1 NZ_CP064405:c2238838-2238533 [Mycobacterium tuberculosis variant bovis BCG]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT181240 NZ_CP085814:c1739229-1738855 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References