Detailed information of TA system TA181200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5525337..5525932
• Replicon: chromosome
• Accession: NZ_CP064403
• Organism: Pseudomonas aeruginosa strain WTJH12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ITG71_RS25715
• Protein ID: WP_003113526.1
• Coordinates: 5525654..5525932 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ITG71_RS25710
• Protein ID: WP_003099268.1
• Coordinates: 5525337..5525642 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG71_RS25670 (ITG71_25655)	5520393..5520869	-	477	WP_003125367.1	putative molybdenum carrier protein	-
ITG71_RS25675 (ITG71_25660)	5520872..5521282	-	411	WP_003125368.1	hypothetical protein	-
ITG71_RS25680 (ITG71_25665)	5521279..5521923	-	645	WP_042956130.1	hypothetical protein	-
ITG71_RS25685 (ITG71_25670)	5521941..5522606	-	666	WP_042956131.1	hypothetical protein	-
ITG71_RS25690 (ITG71_25675)	5522750..5522974	+	225	WP_042956132.1	helix-turn-helix transcriptional regulator	-
ITG71_RS25695 (ITG71_25680)	5523399..5524421	-	1023	WP_003125371.1	replication protein RepA	-
ITG71_RS25700 (ITG71_25685)	5524460..5524672	-	213	WP_003125372.1	AlpA family phage regulatory protein	-
ITG71_RS25710 (ITG71_25695)	5525337..5525642	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
ITG71_RS25715 (ITG71_25700)	5525654..5525932	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG71_RS32895	5525985..5526113	-	129	Protein_5091	integrase	-
ITG71_RS25720 (ITG71_25705)	5526261..5528489	+	2229	WP_003099265.1	TonB-dependent receptor	-
ITG71_RS25725 (ITG71_25710)	5528559..5529206	-	648	WP_003095021.1	carbonate dehydratase	-
ITG71_RS25730 (ITG71_25715)	5529268..5530506	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T181200 WP_003113526.1 NZ_CP064403:c5525932-5525654 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T181200 NZ_CP085811:c1300969-1300751 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT181200 WP_003099268.1 NZ_CP064403:c5525642-5525337 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT181200 NZ_CP085811:c1301371-1300997 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAAC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3