Detailed information of TA system TA181191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5918646..5919241
• Replicon: chromosome
• Accession: NZ_CP064401
• Organism: Pseudomonas aeruginosa strain NDTH10366

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ITG67_RS27760
• Protein ID: WP_003113526.1
• Coordinates: 5918963..5919241 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ITG67_RS27755
• Protein ID: WP_003099268.1
• Coordinates: 5918646..5918951 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG67_RS27720 (ITG67_27735)	5913797..5914645	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
ITG67_RS27730 (ITG67_27745)	5914812..5915753	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
ITG67_RS27735 (ITG67_27750)	5915870..5916484	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
ITG67_RS27740 (ITG67_27755)	5916526..5917110	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
ITG67_RS27745 (ITG67_27760)	5917151..5918251	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
ITG67_RS27755 (ITG67_27770)	5918646..5918951	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
ITG67_RS27760 (ITG67_27775)	5918963..5919241	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG67_RS35105	5919294..5919422	-	129	Protein_5506	integrase	-
ITG67_RS27765 (ITG67_27780)	5919570..5921798	+	2229	WP_003099265.1	TonB-dependent receptor	-
ITG67_RS27770 (ITG67_27785)	5921868..5922515	-	648	WP_003095021.1	carbonate dehydratase	-
ITG67_RS27775 (ITG67_27790)	5922577..5923815	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T181191 WP_003113526.1 NZ_CP064401:c5919241-5918963 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T181191 NZ_CP085810:3838658-3838969 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGCAATTCATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGCAGTGGCGTACGAATTATCTATTTTCATCGTAGTCAACGGTATGAGGTTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT181191 WP_003099268.1 NZ_CP064401:c5918951-5918646 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT181191 NZ_CP085810:3838970-3839260 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: V6ALY3