Detailed information of TA system TA181175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5871138..5871733
• Replicon: chromosome
• Accession: NZ_CP064397
• Organism: Pseudomonas aeruginosa strain SRRSH1002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ITG69_RS27335
• Protein ID: WP_003113526.1
• Coordinates: 5871455..5871733 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ITG69_RS27330
• Protein ID: WP_003099268.1
• Coordinates: 5871138..5871443 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG69_RS27300 (ITG69_27300)	5866184..5866618	-	435	WP_009875803.1	hypothetical protein	-
ITG69_RS27305 (ITG69_27305)	5867134..5867424	-	291	WP_152959653.1	DUF5447 family protein	-
ITG69_RS27310 (ITG69_27310)	5867600..5867872	-	273	WP_058175553.1	hypothetical protein	-
ITG69_RS27315 (ITG69_27315)	5868319..5868654	+	336	WP_058175552.1	hypothetical protein	-
ITG69_RS27320 (ITG69_27320)	5868657..5869358	-	702	WP_152959654.1	retron Ec48 family effector membrane protein	-
ITG69_RS27325 (ITG69_27325)	5869345..5870520	-	1176	WP_071557852.1	reverse transcriptase family protein	-
ITG69_RS27330 (ITG69_27330)	5871138..5871443	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
ITG69_RS27335 (ITG69_27335)	5871455..5871733	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG69_RS32595	5871786..5871914	-	129	Protein_5420	integrase	-
ITG69_RS27340 (ITG69_27340)	5872062..5874290	+	2229	WP_003099265.1	TonB-dependent receptor	-
ITG69_RS27345 (ITG69_27345)	5874360..5875007	-	648	WP_003095021.1	carbonate dehydratase	-
ITG69_RS27350 (ITG69_27350)	5875069..5876307	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T181175 WP_003113526.1 NZ_CP064397:c5871733-5871455 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T181175 NZ_CP085809:434528-435019 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGTGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT181175 WP_003099268.1 NZ_CP064397:c5871443-5871138 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT181175 NZ_CP085809:434238-434531 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGTTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGTGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3