Detailed information of TA system TA181159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5765179..5765774
• Replicon: chromosome
• Accession: NZ_CP064393
• Organism: Pseudomonas aeruginosa strain SRRSH1101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ITG66_RS26865
• Protein ID: WP_003113526.1
• Coordinates: 5765496..5765774 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ITG66_RS26860
• Protein ID: WP_003099268.1
• Coordinates: 5765179..5765484 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG66_RS26830 (ITG66_26835)	5760225..5760659	-	435	WP_009875803.1	hypothetical protein	-
ITG66_RS26835 (ITG66_26840)	5761175..5761465	-	291	WP_152959653.1	DUF5447 family protein	-
ITG66_RS26840 (ITG66_26845)	5761641..5761913	-	273	WP_058175553.1	hypothetical protein	-
ITG66_RS26845 (ITG66_26850)	5762360..5762695	+	336	WP_058175552.1	hypothetical protein	-
ITG66_RS26850 (ITG66_26855)	5762698..5763399	-	702	WP_152959654.1	retron Ec48 family effector membrane protein	-
ITG66_RS26855 (ITG66_26860)	5763386..5764561	-	1176	WP_071557852.1	reverse transcriptase family protein	-
ITG66_RS26860 (ITG66_26865)	5765179..5765484	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
ITG66_RS26865 (ITG66_26870)	5765496..5765774	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG66_RS31985	5765827..5765955	-	129	Protein_5323	integrase	-
ITG66_RS26870 (ITG66_26875)	5766103..5768331	+	2229	WP_003099265.1	TonB-dependent receptor	-
ITG66_RS26875 (ITG66_26880)	5768401..5769048	-	648	WP_003095021.1	carbonate dehydratase	-
ITG66_RS26880 (ITG66_26885)	5769110..5770348	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T181159 WP_003113526.1 NZ_CP064393:c5765774-5765496 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T181159 NZ_CP085807:c4751415-4751041 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT181159 WP_003099268.1 NZ_CP064393:c5765484-5765179 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT181159 NZ_CP085807:c4751657-4751415 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: V6ALY3