Detailed information of TA system TA181151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5585172..5585767
• Replicon: chromosome
• Accession: NZ_CP064392
• Organism: Pseudomonas aeruginosa strain SRRSH15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ITG70_RS26160
• Protein ID: WP_003113526.1
• Coordinates: 5585489..5585767 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ITG70_RS26155
• Protein ID: WP_003113527.1
• Coordinates: 5585172..5585477 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG70_RS26135	5581248..5581505	+	258	WP_010793759.1	ATP-binding protein	-
ITG70_RS26140 (ITG70_26145)	5581881..5582744	-	864	WP_010793758.1	integrase domain-containing protein	-
ITG70_RS26145 (ITG70_26150)	5583346..5584488	-	1143	WP_010793757.1	STY4528 family pathogenicity island replication protein	-
ITG70_RS26155 (ITG70_26160)	5585172..5585477	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
ITG70_RS26160 (ITG70_26165)	5585489..5585767	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG70_RS31520	5585820..5585948	-	129	Protein_5191	integrase	-
ITG70_RS26165 (ITG70_26170)	5586096..5588324	+	2229	WP_003099265.1	TonB-dependent receptor	-
ITG70_RS26170 (ITG70_26175)	5588394..5589041	-	648	WP_003095021.1	carbonate dehydratase	-
ITG70_RS26175 (ITG70_26180)	5589103..5590341	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T181151 WP_003113526.1 NZ_CP064392:c5585767-5585489 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T181151 NZ_CP085807:927355-927753 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTGAAATTCATGCTTGATACCAATACTTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCACCGG
AGCGTAATTTTGCAGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTACCGAACTGGCCCGCAAGGGAACACCTGTCGGGTCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT181151 WP_003113527.1 NZ_CP064392:c5585477-5585172 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT181151 NZ_CP085807:927128-927355 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGCACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3