Detailed information of TA system TA181143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4203481..4204076
• Replicon: chromosome
• Accession: NZ_CP064391
• Organism: Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IUJ43_RS19355
• Protein ID: WP_003113526.1
• Coordinates: 4203798..4204076 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IUJ43_RS19350
• Protein ID: WP_003113527.1
• Coordinates: 4203481..4203786 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ43_RS19320 (IUJ43_19275)	4198585..4198875	-	291	WP_033992920.1	DUF5447 family protein	-
IUJ43_RS19325 (IUJ43_19280)	4199087..4199359	-	273	WP_003115921.1	hypothetical protein	-
IUJ43_RS19330	4199485..4199754	+	270	WP_071536122.1	hypothetical protein	-
IUJ43_RS19335 (IUJ43_19285)	4199889..4200995	+	1107	WP_193218117.1	protein phosphatase 2C domain-containing protein	-
IUJ43_RS19340 (IUJ43_19290)	4200998..4202047	+	1050	WP_003113529.1	serine/threonine-protein kinase	-
IUJ43_RS19345 (IUJ43_19295)	4202116..4203117	+	1002	WP_228772168.1	serine/threonine-protein kinase	-
IUJ43_RS19350 (IUJ43_19300)	4203481..4203786	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
IUJ43_RS19355 (IUJ43_19305)	4203798..4204076	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUJ43_RS19360	4204129..4204257	-	129	Protein_3830	integrase	-
IUJ43_RS19365 (IUJ43_19310)	4204405..4206633	+	2229	WP_003113525.1	TonB-dependent receptor	-
IUJ43_RS19370 (IUJ43_19315)	4206703..4207350	-	648	WP_003095021.1	carbonate dehydratase	-
IUJ43_RS19375 (IUJ43_19320)	4207412..4208650	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T181143 WP_003113526.1 NZ_CP064391:c4204076-4203798 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T181143 NZ_CP085806:c3584812-3584399 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT181143 WP_003113527.1 NZ_CP064391:c4203786-4203481 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT181143 NZ_CP085806:c3585059-3584793 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3