Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4261956..4262755 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag IUJ37_RS21050 Protein ID WP_000347267.1
Coordinates 4261956..4262420 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IUJ37_RS21055 Protein ID WP_001307405.1
Coordinates 4262420..4262755 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS21020 (4256957) 4256957..4257391 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
IUJ37_RS21025 (4257409) 4257409..4258287 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
IUJ37_RS21030 (4258277) 4258277..4259056 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
IUJ37_RS21035 (4259067) 4259067..4259540 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IUJ37_RS21040 (4259563) 4259563..4260843 - 1281 WP_000681935.1 tagatose-bisphosphate aldolase subunit KbaZ -
IUJ37_RS21045 (4261092) 4261092..4261901 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IUJ37_RS21050 (4261956) 4261956..4262420 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IUJ37_RS21055 (4262420) 4262420..4262755 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IUJ37_RS21060 (4262904) 4262904..4264475 - 1572 WP_001273741.1 galactarate dehydratase -
IUJ37_RS21065 (4264849) 4264849..4266183 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IUJ37_RS21070 (4266199) 4266199..4266969 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T181109 WP_000347267.1 NZ_CP064387:c4262420-4261956 [Escherichia coli O104:H4]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T181109 NZ_CP085804:1841600-1841703 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT181109 WP_001307405.1 NZ_CP064387:c4262755-4262420 [Escherichia coli O104:H4]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT181109 NZ_CP085804:c1841705-1841560 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References