Detailed information of TA system TA181106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3405596..3406198
• Replicon: chromosome
• Accession: NZ_CP064387
• Organism: Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IUJ37_RS16825
• Protein ID: WP_000897305.1
• Coordinates: 3405887..3406198 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IUJ37_RS16820
• Protein ID: WP_000356397.1
• Coordinates: 3405596..3405886 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ37_RS16795 (3401540)	3401540..3402442	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IUJ37_RS16800 (3402439)	3402439..3403074	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IUJ37_RS16805 (3403071)	3403071..3404000	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IUJ37_RS16810 (3404330)	3404330..3404572	-	243	WP_001087409.1	protein YiiF	-
IUJ37_RS16815 (3404792)	3404792..3405010	-	219	WP_001315930.1	CopG family transcriptional regulator	-
IUJ37_RS16820 (3405596)	3405596..3405886	-	291	WP_000356397.1	NadS family protein	Antitoxin
IUJ37_RS16825 (3405887)	3405887..3406198	-	312	WP_000897305.1	hypothetical protein	Toxin
IUJ37_RS16830 (3406427)	3406427..3407335	+	909	WP_001387201.1	alpha/beta hydrolase	-
IUJ37_RS16835 (3407399)	3407399..3408340	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IUJ37_RS16840 (3408385)	3408385..3408822	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
IUJ37_RS16845 (3408819)	3408819..3409691	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IUJ37_RS16850 (3409685)	3409685..3410284	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T181106 WP_000897305.1 NZ_CP064387:c3406198-3405887 [Escherichia coli O104:H4]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T181106 NZ_CP085804:547390-547677 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT181106 WP_000356397.1 NZ_CP064387:c3405886-3405596 [Escherichia coli O104:H4]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT181106 NZ_CP085804:547664-547966 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7