Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2504734..2505275 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag IUJ37_RS12610 Protein ID WP_000615974.1
Coordinates 2504997..2505275 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IUJ37_RS12605 Protein ID WP_000729704.1
Coordinates 2504734..2504994 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS12580 (2500517) 2500517..2501302 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
IUJ37_RS12585 (2501274) 2501274..2502986 + 1713 Protein_2471 flagellar biosynthesis protein FlhA -
IUJ37_RS12590 (2503091) 2503091..2503369 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
IUJ37_RS12595 (2503362) 2503362..2503718 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
IUJ37_RS12600 (2503775) 2503775..2504548 - 774 WP_000543895.1 C40 family peptidase -
IUJ37_RS12605 (2504734) 2504734..2504994 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IUJ37_RS12610 (2504997) 2504997..2505275 + 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IUJ37_RS12615 (2505431) 2505431..2506171 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IUJ37_RS12620 (2506142) 2506142..2506909 - 768 WP_000333380.1 class II glutamine amidotransferase -
IUJ37_RS12625 (2507115) 2507115..2507693 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T181102 WP_000615974.1 NZ_CP064387:2504997-2505275 [Escherichia coli O104:H4]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T181102 NZ_CP085804:45847-46158 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT181102 WP_000729704.1 NZ_CP064387:2504734-2504994 [Escherichia coli O104:H4]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT181102 NZ_CP085804:46159-46449 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References