Detailed information of TA system TA181059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 5436519..5437213
• Replicon: chromosome
• Accession: NZ_CP064383
• Organism: Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: IUJ36_RS27085
• Protein ID: WP_001263495.1
• Coordinates: 5436815..5437213 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IUJ36_RS27080
• Protein ID: WP_000554757.1
• Coordinates: 5436519..5436812 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ36_RS27060 (5432230)	5432230..5432727	+	498	Protein_5296	REP-associated tyrosine transposase RayT	-
IUJ36_RS27065 (5432872)	5432872..5434584	-	1713	Protein_5297	flagellar biosynthesis protein FlhA	-
IUJ36_RS27070 (5434556)	5434556..5435341	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
IUJ36_RS27075 (5435412)	5435412..5436467	+	1056	WP_001226188.1	DNA polymerase IV	-
IUJ36_RS27080 (5436519)	5436519..5436812	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IUJ36_RS27085 (5436815)	5436815..5437213	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IUJ36_RS27090 (5437223)	5437223..5437675	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
IUJ36_RS27095 (5437994)	5437994..5438197	+	204	Protein_5303	RtcB family protein	-
IUJ36_RS27100 (5438196)	5438196..5438717	+	522	Protein_5304	peptide chain release factor H	-
IUJ36_RS27105 (5438774)	5438774..5440231	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
IUJ36_RS27110 (5440492)	5440492..5440950	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (5441546)	5441546..5441626	+	81	NuclAT_12	-	-
- (5441546)	5441546..5441626	+	81	NuclAT_12	-	-
- (5441546)	5441546..5441626	+	81	NuclAT_12	-	-
- (5441546)	5441546..5441626	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T181059 WP_001263495.1 NZ_CP064383:5436815-5437213 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T181059 NZ_CP085801:3170375-3170632 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT181059 WP_000554757.1 NZ_CP064383:5436519-5436812 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT181059 NZ_CP085801:c3170550-3170218 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Antitoxin

• AlphaFold DB: S1FJN6