Detailed information of TA system TA181050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3703168..3703967
• Replicon: chromosome
• Accession: NZ_CP064383
• Organism: Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: IUJ36_RS18965
• Protein ID: WP_000347273.1
• Coordinates: 3703503..3703967 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: IUJ36_RS18960
• Protein ID: WP_001302819.1
• Coordinates: 3703168..3703503 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ36_RS18945 (3698953)	3698953..3699723	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
IUJ36_RS18950 (3699739)	3699739..3701073	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
IUJ36_RS18955 (3701448)	3701448..3703019	+	1572	WP_001273776.1	galactarate dehydratase	-
IUJ36_RS18960 (3703168)	3703168..3703503	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IUJ36_RS18965 (3703503)	3703503..3703967	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IUJ36_RS18970 (3704022)	3704022..3704831	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
IUJ36_RS18975 (3705080)	3705080..3706360	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IUJ36_RS18980 (3706383)	3706383..3706856	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IUJ36_RS18985 (3706867)	3706867..3707646	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
IUJ36_RS18990 (3707636)	3707636..3708514	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
IUJ36_RS18995 (3708532)	3708532..3708966	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T181050 WP_000347273.1 NZ_CP064383:3703503-3703967 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T181050 NZ_CP085800:4371942-4372355 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT181050 WP_001302819.1 NZ_CP064383:3703168-3703503 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT181050 NZ_CP085800:4371695-4371961 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V0SSH7