Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3660290..3661017 Replicon chromosome
Accession NZ_CP064383
Organism Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IUJ36_RS18740 Protein ID WP_000550189.1
Coordinates 3660703..3661017 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IUJ36_RS18735 Protein ID WP_000560249.1
Coordinates 3660290..3660706 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ36_RS18725 (3655350) 3655350..3657701 + 2352 WP_000695517.1 alpha-glucosidase -
IUJ36_RS18730 (3658227) 3658227..3660245 + 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IUJ36_RS18735 (3660290) 3660290..3660706 - 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IUJ36_RS18740 (3660703) 3660703..3661017 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IUJ36_RS18745 (3661301) 3661301..3662437 - 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IUJ36_RS18750 (3662522) 3662522..3663025 + 504 WP_001295542.1 M48 family metallopeptidase -
IUJ36_RS18755 (3663102) 3663102..3663794 + 693 WP_000942543.1 vancomycin high temperature exclusion protein -
IUJ36_RS18760 (3663873) 3663873..3664859 + 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T181049 WP_000550189.1 NZ_CP064383:c3661017-3660703 [Escherichia coli O157:H7]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T181049 NZ_CP085800:3905625-3905900 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT181049 WP_000560249.1 NZ_CP064383:c3660706-3660290 [Escherichia coli O157:H7]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT181049 NZ_CP085800:3905362-3905622 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References