Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4548113..4548840 Replicon chromosome
Accession NZ_CP064376
Organism Shigella sonnei strain PartP-Ssonnei-RM8376

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IUJ51_RS22995 Protein ID WP_000550189.1
Coordinates 4548113..4548427 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IUJ51_RS23000 Protein ID WP_000560263.1
Coordinates 4548424..4548840 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ51_RS22975 (4544279) 4544279..4545265 - 987 WP_001446103.1 Gfo/Idh/MocA family oxidoreductase -
IUJ51_RS22980 (4545344) 4545344..4546027 - 684 WP_001183060.1 vancomycin high temperature exclusion protein -
IUJ51_RS22985 (4546104) 4546104..4546607 - 504 WP_001305117.1 M48 family metallopeptidase -
IUJ51_RS22990 (4546692) 4546692..4547828 + 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IUJ51_RS22995 (4548113) 4548113..4548427 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IUJ51_RS23000 (4548424) 4548424..4548840 + 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IUJ51_RS23005 (4548885) 4548885..4550903 - 2019 WP_000121451.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IUJ51_RS23010 (4551129) 4551129..4553480 - 2352 WP_000695472.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T181023 WP_000550189.1 NZ_CP064376:4548113-4548427 [Shigella sonnei]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T181023 NZ_CP085799:2246425-2246643 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT181023 WP_000560263.1 NZ_CP064376:4548424-4548840 [Shigella sonnei]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT181023 NZ_CP085799:2246023-2246397 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References