Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2827568..2828109 Replicon chromosome
Accession NZ_CP064376
Organism Shigella sonnei strain PartP-Ssonnei-RM8376

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IUJ51_RS14565 Protein ID WP_000615976.1
Coordinates 2827831..2828109 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IUJ51_RS14560 Protein ID WP_000729704.1
Coordinates 2827568..2827828 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ51_RS14535 (2823028) 2823028..2824740 + 1713 Protein_2838 flagellar biosynthesis protein FlhA -
IUJ51_RS14540 (2824863) 2824863..2825360 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
IUJ51_RS14545 (2825431) 2825431..2825559 - 129 Protein_2840 ISAs1 family transposase -
IUJ51_RS14550 (2825614) 2825614..2826320 + 707 WP_237163091.1 IS1-like element IS1A family transposase -
IUJ51_RS14555 (2826609) 2826609..2827382 - 774 WP_127148796.1 C40 family peptidase -
IUJ51_RS14560 (2827568) 2827568..2827828 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IUJ51_RS14565 (2827831) 2827831..2828109 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IUJ51_RS14570 (2828265) 2828265..2829005 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IUJ51_RS14575 (2828976) 2828976..2829743 - 768 WP_000333380.1 class II glutamine amidotransferase -
IUJ51_RS14580 (2829949) 2829949..2830527 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2825996..2838970 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T181021 WP_000615976.1 NZ_CP064376:2827831-2828109 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T181021 NZ_CP085799:c1278451-1278296 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT181021 WP_000729704.1 NZ_CP064376:2827568-2827828 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT181021 NZ_CP085799:1278463-1278521 [Salmonella enterica subsp. enterica serovar Enteritidis]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References