Detailed information of TA system TA180904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1140233..1140888
• Replicon: chromosome
• Accession: NZ_CP064354
• Organism: Pseudomonas fragi strain NL20W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IS178_RS05060
• Protein ID: WP_029611525.1
• Coordinates: 1140544..1140888 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS178_RS05055
• Protein ID: WP_016781720.1
• Coordinates: 1140233..1140547 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS178_RS05040	1138107..1139507	+	1401	WP_029611523.1	FAD-binding protein	-
IS178_RS05050	1139838..1140182	+	345	WP_029611524.1	cupin	-
IS178_RS05055	1140233..1140547	-	315	WP_016781720.1	transcriptional regulator	Antitoxin
IS178_RS05060	1140544..1140888	-	345	WP_029611525.1	toxin	Toxin
IS178_RS05070	1141463..1142680	+	1218	WP_029611526.1	MFS transporter	-
IS178_RS05075	1142762..1143286	-	525	WP_016781723.1	RNA polymerase sigma factor	-
IS178_RS05080	1143586..1144647	+	1062	WP_050901294.1	type III secretion system gatekeeper subunit SctW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13187.93 Da
• Isoelectric Point: 8.3658

>T180904 WP_029611525.1 NZ_CP064354:c1140888-1140544 [Pseudomonas fragi]
IRTVFFETTSFTATVGDYLNDDEYRQLQVEMQANPLAGDVMPRTGGFRKLRWQDTRRGKGKRGGLRVIYYWLLDDGQFWM
FAIYDKDELENLTADQEKALKTAIGAELKQRGGK

Nucleotide

• Length: 345 bp

>T180904 NZ_CP085786:34052-34345 [Synechococcus elongatus PCC 6301]
ATGGAGCAGTATGAATGGGACGAACAAAAGCGACTCTCTAACCTAAACAAGCACGGACTGGACTTTCGAGCTGCTGCCCT
TGTGCTGGCCAATCGTTATCGCTTCGACATCACGACCGAGCGAAATCAGGAGCAGCGCATCCAAGCCTTTGCCTATGTCA
TGCAAGTCTTAGCGGTGCTCACAGTGGTCTATCGACCGGGTAAAATTCCGCGAATTATTAGCTTTCGTCCTGCAAGTCGG
ACTGAACGGGAGATTTATTATGAGTGGCTCGAAACGAACCCGCATGACGCTTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11936.91 Da
• Isoelectric Point: 10.0925

>AT180904 WP_016781720.1 NZ_CP064354:c1140547-1140233 [Pseudomonas fragi]
MKKRDLFAELMQGVNEMGEQREGKITLRQYEMEALPAPEVTAAEIVSIRKKLHMSQPVFARQIRTSPDTLKNWEQSKSKP
NAQAALLIKLVERFPDMVDRLNAV

Nucleotide

• Length: 315 bp

>AT180904 NZ_CP085786:34335-34640 [Synechococcus elongatus PCC 6301]
ATGACGCTTGAAGAAATGCAGAATGCGATCGCGGCGAGACAAGATGAAAGCGATTGGGAGGGGGTGCGGCAGCGAATTGA
GGCGGGACTTGATCCAGAGCCAGATGAGGATTCACCCGATGCCACAGAACTGATGCGAGCAGCGCTGCAACAGAAGCGGG
GTAGACCGCCCAGCCTCAATCCCAAAACCCAGATTGCGATCCGCCTCGATCGCGATATCGTCGAGGCTTTTCGGGCGGGT
GGCCCTGGCTGGCAAACCCGCATGAATGCGGCCCTACGAGAATGGCTTGCAGCCCATCCTCATTAG