Detailed information of TA system TA180901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 447227..447890
• Replicon: chromosome
• Accession: NZ_CP064354
• Organism: Pseudomonas fragi strain NL20W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IS178_RS02105
• Protein ID: WP_016783068.1
• Coordinates: 447227..447592 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A494R510
• Locus tag: IS178_RS02110
• Protein ID: WP_016783069.1
• Coordinates: 447585..447890 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS178_RS02090	442447..444525	-	2079	WP_029611677.1	TonB-dependent siderophore receptor	-
IS178_RS02095	444783..445478	+	696	WP_016783066.1	DsbA family protein	-
IS178_RS02100	445728..447011	+	1284	WP_016783067.1	OprD family porin	-
IS178_RS02105	447227..447592	+	366	WP_016783068.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS178_RS02110	447585..447890	+	306	WP_016783069.1	XRE family transcriptional regulator	Antitoxin
IS178_RS02115	447897..448850	-	954	WP_016783070.1	DNA-binding transcriptional regulator DsdC	-
IS178_RS02120	448934..450280	-	1347	WP_016783071.1	D-serine ammonia-lyase	-
IS178_RS02125	450324..451664	-	1341	WP_016783072.1	gluconate:H+ symporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14055.86 Da
• Isoelectric Point: 6.7513

>T180901 WP_016783068.1 NZ_CP064354:447227-447592 [Pseudomonas fragi]
MTWNVEYTDEFGHWWERLSEKEQVSVAASVKLLELLGPALRFPHCSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
LLLIGGDKTGHDRWYEENVPVAQRLYDVHLQTLIEEGQNNG

Nucleotide

• Length: 366 bp

>T180901 NZ_CP085783:c31628-31218 [Enterobacter hormaechei]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11395.13 Da
• Isoelectric Point: 7.2023

>AT180901 WP_016783069.1 NZ_CP064354:447585-447890 [Pseudomonas fragi]
MAKKFSGLEARMSPEARAQAQALYVQHLQEMPLHELRKAQALSQETLAKALNINQAAVSKMERRTDMYISTLRNYIQAMG
GELEIIATFPDGQIKIENFAE

Nucleotide

• Length: 306 bp

>AT180901 NZ_CP085783:c31855-31625 [Enterobacter hormaechei]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A494R510