Detailed information of TA system TA180833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 457341..457908
• Replicon: chromosome
• Accession: NZ_CP064330
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q87EE4
• Locus tag: IUD22_RS01875
• Protein ID: WP_004090695.1
• Coordinates: 457341..457622 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: IUD22_RS01880
• Protein ID: WP_004085172.1
• Coordinates: 457633..457908 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD22_RS01850	453764..454483	-	720	Protein_346	phage late control D family protein	-
IUD22_RS01855	454383..455354	-	972	WP_011097609.1	hypothetical protein	-
IUD22_RS01860	455362..455919	-	558	WP_011097610.1	phage tail protein I	-
IUD22_RS01865	455912..456805	-	894	WP_011097611.1	baseplate J/gp47 family protein	-
IUD22_RS01870	456805..457143	-	339	WP_004090694.1	GPW/gp25 family protein	-
IUD22_RS01875	457341..457622	+	282	WP_004090695.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD22_RS01880	457633..457908	+	276	WP_004085172.1	HigA family addiction module antidote protein	Antitoxin
IUD22_RS01885	457996..458298	+	303	WP_004090696.1	type II toxin-antitoxin system MqsR family toxin	-
IUD22_RS01890	458301..458702	+	402	WP_004090697.1	type II toxin-antitoxin system MqsA family antitoxin	-
IUD22_RS01895	458771..459358	-	588	WP_011097612.1	phage baseplate assembly protein V	-
IUD22_RS01900	459355..459897	-	543	WP_011097613.1	hypothetical protein	-
IUD22_RS01905	459873..460394	-	522	WP_011097614.1	hypothetical protein	-
IUD22_RS01910	460391..460714	-	324	WP_011097935.1	hypothetical protein	-
IUD22_RS01915	460714..460974	-	261	WP_011097616.1	hypothetical protein	-
IUD22_RS01920	460992..462866	-	1875	WP_011097617.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	445939..468772	22833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10639.21 Da
• Isoelectric Point: 10.0980

>T180833 WP_004090695.1 NZ_CP064330:457341-457622 [Xylella fastidiosa subsp. fastidiosa]
MIKSFRHKGIQQFFLKGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T180833 NZ_CP085748:3408669-3408848 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTAGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10072.68 Da
• Isoelectric Point: 10.2184

>AT180833 WP_004085172.1 NZ_CP064330:457633-457908 [Xylella fastidiosa subsp. fastidiosa]
MKRMHNPAHPGEVLREYLGELTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQFKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT180833 NZ_CP085748:3408870-3409280 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCGGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAATACACCGGTGCGGTATGGGCCCTGGTTGAC
GTGGATATAACTCGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTTCCCTCATTGCTGATCCGCCGTATCGATCA
GTTTGTGGCCGCGCATCCAGAATACGGCAGTCGGTCTGGCTTCCTGTCTCGTGTCGCGGCAGATAAAGTAATCGGACGAG
AAAAACGATAA

Structures

Toxin

• AlphaFold DB: Q87EE4