Detailed information of TA system TA180826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 457992..458698
• Replicon: chromosome
• Accession: NZ_CP064328
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-14

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: Q87EE2
• Locus tag: IUD25_RS01865
• Protein ID: WP_004090696.1
• Coordinates: 457992..458294 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q87EE1
• Locus tag: IUD25_RS01870
• Protein ID: WP_004090697.1
• Coordinates: 458297..458698 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD25_RS01830	453761..454480	-	720	Protein_342	phage late control D family protein	-
IUD25_RS01835	454380..455351	-	972	WP_011097609.1	hypothetical protein	-
IUD25_RS01840	455359..455915	-	557	Protein_344	phage tail protein I	-
IUD25_RS01845	455908..456801	-	894	WP_011097611.1	baseplate J/gp47 family protein	-
IUD25_RS01850	456801..457139	-	339	WP_004090694.1	GPW/gp25 family protein	-
IUD25_RS01855	457337..457618	+	282	WP_004090695.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IUD25_RS01860	457629..457904	+	276	WP_004085172.1	HigA family addiction module antidote protein	-
IUD25_RS01865	457992..458294	+	303	WP_004090696.1	type II toxin-antitoxin system MqsR family toxin	Toxin
IUD25_RS01870	458297..458698	+	402	WP_004090697.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
IUD25_RS01875	458767..459354	-	588	WP_011097612.1	phage baseplate assembly protein V	-
IUD25_RS01880	459351..459893	-	543	WP_011097613.1	hypothetical protein	-
IUD25_RS01885	459869..460390	-	522	WP_011097614.1	hypothetical protein	-
IUD25_RS01890	460387..460710	-	324	WP_011097935.1	hypothetical protein	-
IUD25_RS01895	460710..460970	-	261	WP_011097616.1	hypothetical protein	-
IUD25_RS01900	460988..462862	-	1875	WP_011097617.1	phage major capsid protein	-
IUD25_RS01905	462859..463491	-	633	WP_011097618.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	445936..469849	23913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-127)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10948.71 Da
• Isoelectric Point: 8.0251

>T180826 WP_004090696.1 NZ_CP064328:457992-458294 [Xylella fastidiosa subsp. fastidiosa]
MEKGTSHYKLPVVKALVKAGKVRATATAYQGARELGIKDLTGMCTVVLALTSADFYKSMTTYADHTVFQDVYHAKTASGD
EVYLKLTVIDDVLIVSFKEL

Nucleotide

• Length: 303 bp

>T180826 NZ_CP085748:131741-132043 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14944.02 Da
• Isoelectric Point: 7.1650

>AT180826 WP_004090697.1 NZ_CP064328:458297-458698 [Xylella fastidiosa subsp. fastidiosa]
MRCPCCGAAELIHDTRDMLYTYKSETTSIPTVTGDFCPACGEVVLDREHGDRYSELVGLFQRQVNSAYVDPGYITKVRRK
LDLDQRQAAELFGGGVNAFSRYENGKTKPPLSLVKLFKLLDRHPDLLNEVKSS

Nucleotide

• Length: 402 bp

>AT180826 NZ_CP085748:132050-132337 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: Q87EE2

Antitoxin

• AlphaFold DB: Q87EE1