Detailed information of TA system TA180812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1222398..1222965
• Replicon: chromosome
• Accession: NZ_CP064325
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IUD24_RS05305
• Protein ID: WP_004085174.1
• Coordinates: 1222398..1222679 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: IUD24_RS05310
• Protein ID: WP_012337645.1
• Coordinates: 1222690..1222965 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD24_RS05275	1218260..1219852	-	1593	WP_012337641.1	tail fiber domain-containing protein	-
IUD24_RS05280	1219860..1220417	-	558	WP_012337642.1	phage tail protein I	-
IUD24_RS05285	1220410..1221303	-	894	WP_012337643.1	baseplate J/gp47 family protein	-
IUD24_RS05290	1221303..1221641	-	339	WP_004088650.1	GPW/gp25 family protein	-
IUD24_RS05295	1221749..1222030	+	282	WP_004087027.1	XRE family transcriptional regulator	-
IUD24_RS05300	1222042..1222341	+	300	WP_004087025.1	HigA family addiction module antidote protein	-
IUD24_RS05305	1222398..1222679	+	282	WP_004085174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD24_RS05310	1222690..1222965	+	276	WP_012337645.1	HigA family addiction module antidote protein	Antitoxin
IUD24_RS05315	1222970..1223557	-	588	WP_012337646.1	phage baseplate assembly protein V	-
IUD24_RS05320	1223554..1224096	-	543	WP_012337647.1	hypothetical protein	-
IUD24_RS05325	1224072..1224593	-	522	WP_012337648.1	hypothetical protein	-
IUD24_RS05330	1224590..1224913	-	324	WP_012337649.1	hypothetical protein	-
IUD24_RS05335	1224913..1225173	-	261	WP_012337650.1	hypothetical protein	-
IUD24_RS05340	1225191..1227065	-	1875	WP_012337651.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1212106..1263051	50945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 9.8829

>T180812 WP_004085174.1 NZ_CP064325:1222398-1222679 [Xylella fastidiosa subsp. multiplex]
MIKSFRHKGIQQFFLVGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T180812 NZ_CP085743:447215-447700 [Enterobacter hormaechei]
GTGGGGCGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACATTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTTGATGTCTCGTTACGCGGAAACGGGCTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9982.56 Da
• Isoelectric Point: 10.2184

>AT180812 WP_012337645.1 NZ_CP064325:1222690-1222965 [Xylella fastidiosa subsp. multiplex]
MKRMHNPAHPGEVLREYLGDLTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQAKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT180812 NZ_CP085743:446961-447227 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTTATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGGCGTGTAA