Detailed information of TA system TA180807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 459974..460611
• Replicon: chromosome
• Accession: NZ_CP064325
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IUD24_RS01870
• Protein ID: WP_004085174.1
• Coordinates: 460330..460611 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IUD24_RS01865
• Protein ID: WP_004087025.1
• Coordinates: 459974..460273 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD24_RS01840	456192..457784	-	1593	WP_012337641.1	tail fiber domain-containing protein	-
IUD24_RS01845	457792..458349	-	558	WP_012337642.1	phage tail protein I	-
IUD24_RS01850	458342..459235	-	894	WP_012337643.1	baseplate J/gp47 family protein	-
IUD24_RS01855	459235..459573	-	339	WP_004088650.1	GPW/gp25 family protein	-
IUD24_RS01860	459681..459962	+	282	WP_004087027.1	XRE family transcriptional regulator	-
IUD24_RS01865	459974..460273	+	300	WP_004087025.1	HigA family addiction module antidote protein	Antitoxin
IUD24_RS01870	460330..460611	+	282	WP_004085174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD24_RS01875	460622..460897	+	276	WP_012337645.1	HigA family addiction module antidote protein	-
IUD24_RS01880	460902..461489	-	588	WP_012337646.1	phage baseplate assembly protein V	-
IUD24_RS01885	461486..462028	-	543	WP_012337647.1	hypothetical protein	-
IUD24_RS01890	462004..462525	-	522	WP_012337648.1	hypothetical protein	-
IUD24_RS01895	462522..462845	-	324	WP_012337649.1	hypothetical protein	-
IUD24_RS01900	462823..463104	-	282	WP_194957557.1	hypothetical protein	-
IUD24_RS01905	463122..464995	-	1874	Protein_357	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	446878..481250	34372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 9.8829

>T180807 WP_004085174.1 NZ_CP064325:460330-460611 [Xylella fastidiosa subsp. multiplex]
MIKSFRHKGIQQFFLVGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T180807 NZ_CP085740:c5294701-5294318 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11022.77 Da
• Isoelectric Point: 9.0879

>AT180807 WP_004087025.1 NZ_CP064325:459974-460273 [Xylella fastidiosa subsp. multiplex]
MTMSKTLPPIHPGEILREEFMVPLGLSSNALAKAIGVTAARINEIVRERRGITAETALRLARYFGTDPQSWLNLEQHYAL
ECAKRDLGDALSRIHPRVA

Nucleotide

• Length: 300 bp

>AT180807 NZ_CP085740:c5294943-5294701 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA