Detailed information of TA system TA180795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 25188..25704
• Replicon: plasmid pMGB0245-1
• Accession: NZ_CP064300
• Organism: Lacticaseibacillus paracasei subsp. tolerans strain MGB0245

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: LacP0245_RS14900
• Protein ID: WP_016369697.1
• Coordinates: 25423..25704 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: LacP0245_RS14895
• Protein ID: WP_016369696.1
• Coordinates: 25188..25430 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LacP0245_RS14870	20367..21038	-	672	WP_126352983.1	phosphatase PAP2 family protein	-
LacP0245_RS14875	21028..21678	-	651	WP_002819944.1	VTT domain-containing protein	-
LacP0245_RS14880	21896..23254	-	1359	WP_194959276.1	HAMP domain-containing histidine kinase	-
LacP0245_RS14885	23264..23959	-	696	WP_003572018.1	response regulator transcription factor	-
LacP0245_RS14890	24210..24996	-	787	Protein_30	IS5 family transposase	-
LacP0245_RS14895	25188..25430	+	243	WP_016369696.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LacP0245_RS14900	25423..25704	+	282	WP_016369697.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LacP0245_RS14905	25841..26491	+	651	WP_003576925.1	recombinase family protein	-
LacP0245_RS14910	26497..26781	+	285	WP_042745859.1	BRCT domain-containing protein	-
LacP0245_RS14915	27518..27733	+	216	WP_003592982.1	hypothetical protein	-
LacP0245_RS14920	27857..28255	+	399	WP_003597456.1	pyridoxamine 5'-phosphate oxidase family protein	-
LacP0245_RS14925	28350..28847	-	498	Protein_37	transposase	-
LacP0245_RS14930	28982..29323	+	342	Protein_38	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..72374	72374
	-	flank	IS/Tn	-	-	28982..29434	452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-63)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.94 Da
• Isoelectric Point: 9.1895

>T180795 WP_016369697.1 NZ_CP064300:25423-25704 [Lacticaseibacillus paracasei subsp. tolerans]
MNNFIYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T180795 NZ_CP085732:71952-72377 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9244.47 Da
• Isoelectric Point: 5.1548

>AT180795 WP_016369696.1 NZ_CP064300:25188-25430 [Lacticaseibacillus paracasei subsp. tolerans]
MTSQVHFRMSEKEKKDFEIVLNRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT180795 NZ_CP085732:71725-71955 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA