Detailed information of TA system TA180782

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16825..17078
• Replicon: plasmid pJX10-2
• Accession: NZ_CP064260
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IS497_RS29185
• Protein ID: WP_001312851.1
• Coordinates: 16929..17078 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	16825..16884 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS497_RS29145	12207..12551	-	345	Protein_22	IS6-like element IS26 family transposase	-
IS497_RS29150	12603..13307	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS497_RS29155	13371..13532	+	162	Protein_24	DNA helicase	-
IS497_RS29160	13552..14298	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IS497_RS29165	14353..14913	+	561	WP_000139328.1	fertility inhibition protein FinO	-
IS497_RS29170	15045..15245	+	201	WP_015059022.1	hypothetical protein	-
IS497_RS29175	15631..16230	+	600	WP_032083981.1	hypothetical protein	-
IS497_RS29180	16394..16624	+	231	WP_001736714.1	hypothetical protein	-
-	16825..16884	-	60	NuclAT_0	-	Antitoxin
-	16825..16884	-	60	NuclAT_0	-	Antitoxin
-	16825..16884	-	60	NuclAT_0	-	Antitoxin
-	16825..16884	-	60	NuclAT_0	-	Antitoxin
IS497_RS29185	16929..17078	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IS497_RS29190	17362..17610	+	249	WP_000083837.1	replication regulatory protein RepA	-
IS497_RS29195	17855..17929	+	75	WP_001375168.1	RepA leader peptide Tap	-
IS497_RS29200	17922..18779	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IS497_RS29205	19718..20371	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IS497_RS29210	20464..20721	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IS497_RS29215	20654..21055	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IS497_RS29220	21304..21719	+	416	Protein_37	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / blaCTX-M-14 / qacE / cmlA1	-	1..90714	90714
	-	inside	IScluster/Tn	blaTEM-1B / blaCTX-M-14 / qacE / cmlA1	-	21304..54789	33485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T180782 WP_001312851.1 NZ_CP064260:16929-17078 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T180782 NZ_CP085717:337234-337584 [Peribacillus asahii]
ATTGTTGTTAAGCGTGGTGACGTATACTTCGCAGATCTTTCTCCAGTTGTCGGCTCAGAACAAGGTGGAACCCGTCCGGT
ACTCATTCTTCAAAATGATATCGGAAATCGCTTTAGTCCGACAGTGATTGTGGCAGCCATTACAGCTCAAATTCAAAAAG
CAAAATTGCCAACACATGTGGAGATTAACGCGAAAAAATATGGATTCGAGCGAGATTCCGTTATTCTTTTAGAACAAATT
CGCACGATTGATAAGCAACGCTTGACAGACAAAATTACGTATCTCGATGAAACAATGATGCAAAAAGTAAACGAAGCTTT
ACAGATTAGTTTAGGTTTGAAAGAATTTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT180782 NZ_CP064260:c16884-16825 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin