Detailed information of TA system TA180776

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 352222..352648
• Replicon: plasmid pJX10-1
• Accession: NZ_CP064259
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS497_RS28755
• Protein ID: WP_001312861.1
• Coordinates: 352222..352380 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	352424..352648 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS497_RS28730	347826..348009	-	184	Protein_364	small membrane protein	-
IS497_RS28735	348029..348640	-	612	Protein_365	mucoid phenotype A regulator RmpA	-
IS497_RS28740	348772..350328	-	1557	WP_053897648.1	PAS domain-containing methyl-accepting chemotaxis protein	-
IS497_RS28745	350353..351057	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS497_RS28750	351607..351984	+	378	Protein_368	hypothetical protein	-
IS497_RS28755	352222..352380	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	352424..352648	-	225	NuclAT_0	-	Antitoxin
-	352424..352648	-	225	NuclAT_0	-	Antitoxin
-	352424..352648	-	225	NuclAT_0	-	Antitoxin
-	352424..352648	-	225	NuclAT_0	-	Antitoxin
IS497_RS28760	352660..353379	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
IS497_RS28765	353376..353810	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS497_RS28770	353879..355902	-	2024	Protein_372	ParB/RepB/Spo0J family partition protein	-
IS497_RS28775	355963..356196	-	234	WP_000006003.1	DUF905 family protein	-
IS497_RS28780	356254..356781	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA	1..394328	394328
	-	flank	IS/Tn	-	-	350353..351057	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180776 WP_001312861.1 NZ_CP064259:c352380-352222 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180776 NZ_CP085704:4315198-4315491 [Pseudomonas sp. HN2-3]
ATGACTGAAACATTTTTTGACTTCGATGCGGCTGATTACCTGACCACCCCGCAAGCCATCGCGCAGTTCATGTCCGATGC
GCTTGAAACGGGCGATGCCAGCTACGTGGCCAAGGCCGTTGGTGTGGTAGCACGTGCCAAAGGCATGAGCGAATTGGCGA
AAGAGACGGGTTTGTCACGTGAACAACTTTATCGCTCGTTCAGCGAGCACGGTAATCCGACGTTGAAGTCGTTTCTTGCC
GTCATGAAGGCGCTGGGAATAGACATGACCGCCAAACCTCATCTTGCCCACTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT180776 NZ_CP064259:c352648-352424 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin