Detailed information of TA system TA180774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 295581..296308
• Replicon: plasmid pJX10-1
• Accession: NZ_CP064259
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS497_RS28440
• Protein ID: WP_011251285.1
• Coordinates: 295997..296308 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS497_RS28435
• Protein ID: WP_011251286.1
• Coordinates: 295581..296000 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS497_RS28405	290749..291219	-	471	WP_048333570.1	hypothetical protein	-
IS497_RS28410	291412..292167	-	756	Protein_300	hypothetical protein	-
IS497_RS28415	292763..293206	+	444	WP_048333569.1	hypothetical protein	-
IS497_RS28420	293227..294015	+	789	WP_040217257.1	hypothetical protein	-
IS497_RS28425	294029..294364	+	336	Protein_303	conjugal transfer protein TraI	-
IS497_RS28430	294466..295434	-	969	WP_074428168.1	IS5 family transposase	-
IS497_RS28435	295581..296000	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IS497_RS28440	295997..296308	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS497_RS28445	296513..296971	-	459	Protein_307	DDE-type integrase/transposase/recombinase	-
IS497_RS28450	297085..297782	+	698	Protein_308	IS1 family transposase	-
IS497_RS28455	297799..298233	+	435	Protein_309	thioredoxin fold domain-containing protein	-
IS497_RS28460	298250..298561	+	312	WP_011251282.1	hypothetical protein	-
IS497_RS28465	298575..298916	+	342	WP_011251281.1	hypothetical protein	-
IS497_RS28470	298976..299932	+	957	WP_011251280.1	DsbA family protein	-
IS497_RS28475	300357..300797	-	441	WP_011251275.1	hypothetical protein	-
IS497_RS28480	300787..301297	-	511	Protein_314	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA	1..394328	394328
	-	inside	IScluster/Tn	-	-	273256..303217	29961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180774 WP_011251285.1 NZ_CP064259:c296308-295997 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180774 NZ_CP085704:c4042052-4041756 [Pseudomonas sp. HN2-3]
ATGGAAAAGAGAACGCCTCATTGCCCGCTGGAGCGGATAAAAGCCCTGACTGCGGCAAGGCGCGTCAGCCCTACGGGCAC
TGCATTAAGAGATGCTAAGGCGCTAGGAATGGATTTTCCGGGAATGCTTGAGGTAATCACTAGCTTGAAGCGCACTGATT
TCTACAAAAGCATGACCAGCCATATTGATCACCGGGTTTGGCAGGACGTATACCGCCCGTTTACAGCGGTTGGCTACGTT
TACCTGAAAGTGTCCGTGGTCAACGACGTGCTTATCGTGTCCTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT180774 WP_011251286.1 NZ_CP064259:c296000-295581 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180774 NZ_CP085704:c4041753-4041352 [Pseudomonas sp. HN2-3]
ATGAGATGTCCAATTTGCGGGGGTGCCGAGCTTGCACCTGACACTCAGGGCATGCCTTATCGCTACAAGGGAGAGACGAC
CCTGATCCCGGACGTAAGTGGCGATTATTGTTCCGCATGCGGTGAGTGCGTGCTGAGCCATGATCAAGCCATGCGTGTCA
GCGATCTGATGACGGCATTCGAGCGCCAAGTCAATGCAACTGTAGTGGACCCTTCTTTCATTGCCTCCATACGCAGGAAG
TTCGACCTCGATCAGCGGCAAGCGGGGGAAATTTTTGGTGGCGGGGTCAATGCGTTCTCCCGTTATGAAAATGGCAAGAC
CACGCCACCCGTGGCGCTGGTAAAGCTGCTGAAGCTACTGGATCGCCACCCCGAACTGTTCGAGGAAGTGCGTACAGCCT
GA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1