Detailed information of TA system TA180749

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102465..102718
• Replicon: plasmid pJX1-1
• Accession: NZ_CP064253
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IS496_RS27625
• Protein ID: WP_001312851.1
• Coordinates: 102465..102614 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	102659..102718 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS496_RS27590	97824..98239	-	416	Protein_118	IS1 family transposase	-
IS496_RS27595	98488..98889	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IS496_RS27600	98822..99079	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IS496_RS27605	99172..99825	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IS496_RS27610	100764..101621	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IS496_RS27615	101614..101688	-	75	WP_001375168.1	RepA leader peptide Tap	-
IS496_RS27620	101933..102181	-	249	WP_000083837.1	replication regulatory protein RepA	-
IS496_RS27625	102465..102614	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	102659..102718	+	60	NuclAT_1	-	Antitoxin
-	102659..102718	+	60	NuclAT_1	-	Antitoxin
-	102659..102718	+	60	NuclAT_1	-	Antitoxin
-	102659..102718	+	60	NuclAT_1	-	Antitoxin
IS496_RS27630	102919..103149	-	231	WP_001736714.1	hypothetical protein	-
IS496_RS27635	103313..103912	-	600	WP_032083981.1	hypothetical protein	-
IS496_RS27640	104298..104498	-	201	WP_015059022.1	hypothetical protein	-
IS496_RS27645	104630..105190	-	561	WP_000139328.1	fertility inhibition protein FinO	-
IS496_RS27650	105245..105991	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IS496_RS27655	106056..106760	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS496_RS27660	106813..106878	+	66	Protein_132	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2	-	1..167834	167834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T180749 WP_001312851.1 NZ_CP064253:c102614-102465 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T180749 NZ_CP085696:2009765-2009868 [Salmonella enterica subsp. enterica serovar Saintpaul]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 60 bp

>AT180749 NZ_CP064253:102659-102718 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin