Detailed information of TA system TA180745

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64215..64641
• Replicon: plasmid pJX1-1
• Accession: NZ_CP064253
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS496_RS27375
• Protein ID: WP_001312861.1
• Coordinates: 64215..64373 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	64417..64641 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS496_RS27340	59270..59497	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
IS496_RS27345	59591..60277	-	687	WP_015059009.1	PAS domain-containing protein	-
IS496_RS27350	60468..60851	-	384	WP_000124981.1	relaxosome protein TraM	-
IS496_RS27355	61128..61775	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IS496_RS27360	62072..62893	-	822	WP_001234445.1	DUF945 domain-containing protein	-
IS496_RS27365	63004..63300	-	297	WP_001272251.1	hypothetical protein	-
IS496_RS27370	63600..63977	+	378	Protein_74	hypothetical protein	-
IS496_RS27375	64215..64373	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	64417..64641	-	225	NuclAT_0	-	Antitoxin
-	64417..64641	-	225	NuclAT_0	-	Antitoxin
-	64417..64641	-	225	NuclAT_0	-	Antitoxin
-	64417..64641	-	225	NuclAT_0	-	Antitoxin
IS496_RS27380	64653..65372	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
IS496_RS27385	65369..65803	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS496_RS27390	65872..67895	-	2024	Protein_78	ParB/RepB/Spo0J family partition protein	-
IS496_RS27395	67956..68189	-	234	WP_000006003.1	DUF905 family protein	-
IS496_RS27400	68247..68774	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2	-	1..167834	167834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180745 WP_001312861.1 NZ_CP064253:c64373-64215 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180745 NZ_CP085696:359208-359483 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGGGGCAAAGGAAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGGGCCTGACTGGATTCTTATTGATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT180745 NZ_CP064253:c64641-64417 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin