Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 329931..330517 Replicon chromosome
Accession NZ_CP064252
Organism Klebsiella pneumoniae strain JX-CR-hvKP-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag IS496_RS01540 Protein ID WP_002920800.1
Coordinates 330149..330517 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag IS496_RS01535 Protein ID WP_002920802.1
Coordinates 329931..330152 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS496_RS01515 326088..327014 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IS496_RS01520 327011..328288 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
IS496_RS01525 328285..329052 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
IS496_RS01530 329054..329767 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
IS496_RS01535 329931..330152 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IS496_RS01540 330149..330517 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IS496_RS01545 330790..332106 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
IS496_RS01550 332213..333100 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
IS496_RS01555 333097..333942 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
IS496_RS01560 333944..335014 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327011..335751 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T180726 WP_002920800.1 NZ_CP064252:330149-330517 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T180726 NZ_CP085684:860321-860719 [Pseudomonas ficuserectae]
ATGGAATTAATCTTCTTTCCATTTGAACGTGTCATTGAGATAAACACTCAGATCTTGACTACCGAGCCCGGCATGAAGGG
GGCGGTAAATATTCCGACCTTGCAAGGTGCGCTGGCTCGAATCGATAATGCGATCGCCTATAATGGCCTTGACGATGTAT
TTGAAATTGCCGCCGAGTATACTGCCAGTATAGCGCAAGGCCACGCACTGCCTGATGCTAACAAGCGCACCGGACTTGCA
GTTGGCTTGGAGTACCTTTCGCTCAATGATTATGAAATTACTCTAGATAATGATTTGATAGCGGATGCCGTTAGGGACTT
GGTTGTTGGTGATATCAACGAGCATGATTTCGCTGATATTCTTTACTCCTGCTTCATCGCTTCTCAAAATCAGGTTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT180726 WP_002920802.1 NZ_CP064252:329931-330152 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT180726 NZ_CP085684:860151-860321 [Pseudomonas ficuserectae]
ATGAACCACAAGCTGAACACCTATGGCGTATCCATAGTAGAGAGGCCTAAAGTTAAAGCCATCAAGAAGCTAGACTTGGG
TGGCGACTCGGGTAAGCAGATCGTTTATTCAGAAACCAAGCTCGTTCTCAGGACTCACAAGAAGACCTTTAAAAAGTTGG
CTGACATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References