Detailed information of TA system TA180721

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38979..39248
• Replicon: plasmid pJX2-2
• Accession: NZ_CP064248
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS498_RS28770
• Protein ID: WP_001312861.1
• Coordinates: 39090..39248 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	38979..39044 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS498_RS28745	34689..35216	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IS498_RS28750	35274..35507	+	234	WP_000006003.1	DUF905 family protein	-
IS498_RS28755	35568..37591	+	2024	Protein_49	ParB/RepB/Spo0J family partition protein	-
IS498_RS28760	37660..38094	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS498_RS28765	38091..38810	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	38822..39046	+	225	NuclAT_0	-	-
-	38822..39046	+	225	NuclAT_0	-	-
-	38822..39046	+	225	NuclAT_0	-	-
-	38822..39046	+	225	NuclAT_0	-	-
-	38979..39044	-	66	-	-	Antitoxin
IS498_RS28770	39090..39248	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IS498_RS28775	39486..39863	-	378	Protein_53	hypothetical protein	-
IS498_RS28780	40163..40459	+	297	WP_001272251.1	hypothetical protein	-
IS498_RS28785	40570..41391	+	822	WP_001234445.1	DUF945 domain-containing protein	-
IS498_RS28790	41688..42335	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IS498_RS28795	42612..42995	+	384	WP_000124981.1	relaxosome protein TraM	-
IS498_RS28800	43186..43872	+	687	WP_015059009.1	PAS domain-containing protein	-
IS498_RS28805	43966..44193	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..168025	168025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180721 WP_001312861.1 NZ_CP064248:39090-39248 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180721 NZ_CP085638:c3716642-3716235 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGTTTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT180721 NZ_CP064248:c39044-38979 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin