Detailed information of TA system TA180717

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 739..992
• Replicon: plasmid pJX2-2
• Accession: NZ_CP064248
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IS498_RS28520
• Protein ID: WP_001312851.1
• Coordinates: 843..992 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	739..798 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS498_RS28515	308..538	+	231	WP_001736714.1	hypothetical protein	-
-	739..798	-	60	NuclAT_1	-	Antitoxin
-	739..798	-	60	NuclAT_1	-	Antitoxin
-	739..798	-	60	NuclAT_1	-	Antitoxin
-	739..798	-	60	NuclAT_1	-	Antitoxin
IS498_RS28520	843..992	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IS498_RS28525	1276..1524	+	249	WP_000083837.1	replication regulatory protein RepA	-
IS498_RS28530	1769..1843	+	75	WP_001375168.1	RepA leader peptide Tap	-
IS498_RS28535	1836..2693	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IS498_RS28540	3632..4285	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IS498_RS28545	4378..4635	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IS498_RS28550	4568..4969	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IS498_RS28555	5218..5633	+	416	Protein_9	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..168025	168025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T180717 WP_001312851.1 NZ_CP064248:843-992 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T180717 NZ_CP085638:c2806977-2806723 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT180717 NZ_CP064248:c798-739 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin