180716

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 148286..149013 Replicon plasmid pJX2-1
Accession NZ_CP064247
Organism Klebsiella pneumoniae strain JX-CR-hvKP-2

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag IS498_RS27895 Protein ID WP_004118600.1
Coordinates 148702..149013 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag IS498_RS27890 Protein ID WP_000990392.1
Coordinates 148286..148705 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IS498_RS27860 143973..144335 - 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -
IS498_RS27865 144383..144736 - 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
IS498_RS27870 145052..145645 + 594 WP_044348674.1 hypothetical protein -
IS498_RS27875 146069..146728 + 660 WP_000078540.1 hypothetical protein -
IS498_RS27880 146725..147054 + 330 WP_004118613.1 hypothetical protein -
IS498_RS27885 147047..148249 + 1203 WP_003031541.1 hypothetical protein -
IS498_RS27890 148286..148705 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
IS498_RS27895 148702..149013 - 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
IS498_RS27900 149224..149604 + 381 Protein_176 gluconate permease -
IS498_RS27905 149654..150349 - 696 WP_004118595.1 lactate utilization protein C -
IS498_RS27910 150342..151769 - 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
IS498_RS27915 151780..152499 - 720 WP_001102107.1 (Fe-S)-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / aac(3)-IId / blaLAP-2 / qnrS1 / sul1 / qacE / dfrA1 / tet(A) / catA2 / aph(6)-Id / aph(3'')-Ib / sul2 - 1..272092 272092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T180716 WP_004118600.1 NZ_CP064247:c149013-148702 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T180716 NZ_CP085638:c2634305-2634202 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT180716 WP_000990392.1 NZ_CP064247:c148705-148286 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT180716 NZ_CP085638:2634164-2634435 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References