Detailed information of TA system TA180692

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 133194..133620
• Replicon: plasmid pJX3-2
• Accession: NZ_CP064243
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS502_RS28425
• Protein ID: WP_001312861.1
• Coordinates: 133462..133620 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	133194..133418 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS502_RS28400	129061..129588	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IS502_RS28405	129646..129879	+	234	WP_000006003.1	DUF905 family protein	-
IS502_RS28410	129940..131963	+	2024	Protein_168	ParB/RepB/Spo0J family partition protein	-
IS502_RS28415	132032..132466	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS502_RS28420	132463..133182	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	133194..133418	+	225	NuclAT_0	-	Antitoxin
-	133194..133418	+	225	NuclAT_0	-	Antitoxin
-	133194..133418	+	225	NuclAT_0	-	Antitoxin
-	133194..133418	+	225	NuclAT_0	-	Antitoxin
IS502_RS28425	133462..133620	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IS502_RS28430	133858..134235	-	378	Protein_172	hypothetical protein	-
IS502_RS28435	134535..134831	+	297	WP_001272251.1	hypothetical protein	-
IS502_RS28440	134942..135763	+	822	WP_001234445.1	DUF945 domain-containing protein	-
IS502_RS28445	136060..136707	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IS502_RS28450	136984..137367	+	384	WP_000124981.1	relaxosome protein TraM	-
IS502_RS28455	137558..138244	+	687	WP_015059009.1	PAS domain-containing protein	-
IS502_RS28460	138338..138565	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / blaSHV-182	-	1..154868	154868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180692 WP_001312861.1 NZ_CP064243:133462-133620 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180692 NZ_CP085637:1017957-1018292 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT180692 NZ_CP064243:133194-133418 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin