Detailed information of TA system TA180689

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80155..80408
• Replicon: plasmid pJX3-2
• Accession: NZ_CP064243
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IS502_RS28095
• Protein ID: WP_001312851.1
• Coordinates: 80259..80408 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	80155..80214 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS502_RS28055	75537..75881	-	345	Protein_97	IS6-like element IS26 family transposase	-
IS502_RS28060	75933..76637	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS502_RS28065	76701..76862	+	162	Protein_99	DNA helicase	-
IS502_RS28070	76882..77628	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IS502_RS28075	77683..78243	+	561	WP_000139328.1	fertility inhibition protein FinO	-
IS502_RS28080	78375..78575	+	201	WP_015059022.1	hypothetical protein	-
IS502_RS28085	78961..79560	+	600	WP_032083981.1	hypothetical protein	-
IS502_RS28090	79724..79954	+	231	WP_001736714.1	hypothetical protein	-
-	80155..80214	-	60	NuclAT_1	-	Antitoxin
-	80155..80214	-	60	NuclAT_1	-	Antitoxin
-	80155..80214	-	60	NuclAT_1	-	Antitoxin
-	80155..80214	-	60	NuclAT_1	-	Antitoxin
IS502_RS28095	80259..80408	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IS502_RS28100	80692..80940	+	249	WP_000083837.1	replication regulatory protein RepA	-
IS502_RS28105	81185..81259	+	75	WP_001375168.1	RepA leader peptide Tap	-
IS502_RS28110	81252..82109	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IS502_RS28115	83048..83701	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IS502_RS28120	83794..84051	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IS502_RS28125	83984..84385	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IS502_RS28130	84634..85049	+	416	Protein_112	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / blaSHV-182	-	1..154868	154868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T180689 WP_001312851.1 NZ_CP064243:80259-80408 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T180689 NZ_CP085637:273548-273823 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT180689 NZ_CP064243:c80214-80155 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin