Detailed information of TA system TA180687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 154740..155467
• Replicon: plasmid pJX3-1
• Accession: NZ_CP064242
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS502_RS27250
• Protein ID: WP_011251285.1
• Coordinates: 154740..155051 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS502_RS27255
• Protein ID: WP_011251286.1
• Coordinates: 155048..155467 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS502_RS27210	149751..150261	+	511	Protein_166	hypothetical protein	-
IS502_RS27215	150251..150691	+	441	WP_011251275.1	hypothetical protein	-
IS502_RS27220	151116..152072	-	957	WP_011251280.1	DsbA family protein	-
IS502_RS27225	152132..152473	-	342	WP_011251281.1	hypothetical protein	-
IS502_RS27230	152487..152798	-	312	WP_011251282.1	hypothetical protein	-
IS502_RS27235	152815..153249	-	435	Protein_171	thioredoxin fold domain-containing protein	-
IS502_RS27240	153266..153963	-	698	Protein_172	IS1 family transposase	-
IS502_RS27245	154077..154535	+	459	Protein_173	DDE-type integrase/transposase/recombinase	-
IS502_RS27250	154740..155051	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS502_RS27255	155048..155467	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IS502_RS27260	155614..156582	+	969	WP_074428168.1	IS5 family transposase	-
IS502_RS27265	156684..157019	-	336	Protein_177	conjugal transfer protein TraI	-
IS502_RS27270	157033..157821	-	789	WP_040217257.1	hypothetical protein	-
IS502_RS27275	157842..158285	-	444	WP_048333569.1	hypothetical protein	-
IS502_RS27280	158881..159636	+	756	Protein_180	hypothetical protein	-
IS502_RS27285	159829..160299	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN	1..221242	221242
	-	inside	IScluster/Tn	-	-	147832..172138	24306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180687 WP_011251285.1 NZ_CP064242:154740-155051 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180687 NZ_CP085636:c6348406-6348146 [Shewanella sp. MTB7]
ATGAATATTCTCTTCTCTGATAATGCTTGGAACGATTACCTTTATTGGCAACAACAAGATAAAAAAACATTGAAACGAAT
TAACCTGCTTATTAAAGATTGTCAAAGGACACCTTTTGAAGGCATTGGCAAACCAGAAAGATTAAAAGAAAACCTTTCTG
GTTATTGGTCTAGACGGATCACAGATGAACATCGTTTGGTTTATAAGGCAACTCAGGATAGGTTAACGATTGTTGCCCTA
CGCTATCACTACTCTTCCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT180687 WP_011251286.1 NZ_CP064242:155048-155467 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180687 NZ_CP085636:c6348657-6348406 [Shewanella sp. MTB7]
ATGAGTGTTTATAGTTTTACTGAGGTAAGAAGTAATTTTAAGTCTGTTTGTGACAGTGTCGTCAATGACTGCGAACCTGC
AGTGATACATCGTAGAGGTGCTGAAAATGTCGTCATGTTCTCTGAGTCAGAGTTCAATAGTTGGAAAGAGACTATTTACT
TACTTTCAAACCCAGTAAATGCAAAACATCTGCTAGATTCTATAAAGCAGGTTGAAGAAGGCCAGGCTCAAGAGCGCGAG
TTACTGGATTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1