Detailed information of TA system TA180657

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49171..49597
• Replicon: plasmid pJX4-2
• Accession: NZ_CP064237
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS499_RS28465
• Protein ID: WP_001312861.1
• Coordinates: 49171..49329 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49373..49597 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS499_RS28430	44226..44453	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
IS499_RS28435	44547..45233	-	687	WP_015059009.1	PAS domain-containing protein	-
IS499_RS28440	45424..45807	-	384	WP_000124981.1	relaxosome protein TraM	-
IS499_RS28445	46084..46731	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IS499_RS28450	47028..47849	-	822	WP_001234445.1	DUF945 domain-containing protein	-
IS499_RS28455	47960..48256	-	297	WP_001272251.1	hypothetical protein	-
IS499_RS28460	48556..48933	+	378	Protein_61	hypothetical protein	-
IS499_RS28465	49171..49329	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49373..49597	-	225	NuclAT_0	-	Antitoxin
-	49373..49597	-	225	NuclAT_0	-	Antitoxin
-	49373..49597	-	225	NuclAT_0	-	Antitoxin
-	49373..49597	-	225	NuclAT_0	-	Antitoxin
IS499_RS28470	49609..50328	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
IS499_RS28475	50325..50759	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS499_RS28480	50828..52851	-	2024	Protein_65	ParB/RepB/Spo0J family partition protein	-
IS499_RS28485	52912..53145	-	234	WP_000006003.1	DUF905 family protein	-
IS499_RS28490	53203..53730	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	cmlA1 / blaCTX-M-14 / blaSHV-12 / blaKPC-2 / blaTEM-1B	-	1..165027	165027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180657 WP_001312861.1 NZ_CP064237:c49329-49171 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180657 NZ_CP085627:254027-254416 [Erwinia rhapontici]
ATGCCGACATATATGACTCCGGAGTTTGATCGAGACAGAAAGCGTTTTCGTATTAGTGACCAGAAACTCTGCAAGGCCGC
AGATCTGGTCAGAAATGGTGCTCACGATGGGCATTTAGGCCGCAGCGTATATAAGAAACGTATTGGTGTTGCCGGTAGTG
GCGCCAGTAATGGTGCTCGCTCCATCATCTTTTTTTCTCATGGTGATCACCTCTATTTTTTCGATATCTATGTGAAAAGT
GAGCTATCTAAAAAGAAGGGAAAAGAGCTGGAAATGGACGAGATAGAGTCCTACTGCGATGTGGCTCAGGATTTTTTGAA
AATGAACGATTATCTCAGGCAACAGCTGATTAAAGTGAAAGAACTCATTGAGGTAAATTGCGATGACTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT180657 NZ_CP064237:c49597-49373 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin