Detailed information of TA system TA180652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 110549..111276
• Replicon: plasmid pJX4-1
• Accession: NZ_CP064236
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS499_RS27540
• Protein ID: WP_011251285.1
• Coordinates: 110965..111276 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS499_RS27535
• Protein ID: WP_011251286.1
• Coordinates: 110549..110968 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS499_RS27505	105717..106187	-	471	WP_048333570.1	hypothetical protein	-
IS499_RS27510	106380..107135	-	756	Protein_106	hypothetical protein	-
IS499_RS27515	107731..108174	+	444	WP_048333569.1	hypothetical protein	-
IS499_RS27520	108195..108983	+	789	WP_040217257.1	hypothetical protein	-
IS499_RS27525	108997..109332	+	336	Protein_109	conjugal transfer protein TraI	-
IS499_RS27530	109434..110402	-	969	WP_074428168.1	IS5 family transposase	-
IS499_RS27535	110549..110968	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IS499_RS27540	110965..111276	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS499_RS27545	111481..111939	-	459	Protein_113	DDE-type integrase/transposase/recombinase	-
IS499_RS27550	112053..112750	+	698	Protein_114	IS1 family transposase	-
IS499_RS27555	112767..113201	+	435	Protein_115	thioredoxin fold domain-containing protein	-
IS499_RS27560	113218..113529	+	312	WP_011251282.1	hypothetical protein	-
IS499_RS27565	113543..113884	+	342	WP_011251281.1	hypothetical protein	-
IS499_RS27570	113944..114900	+	957	WP_011251280.1	DsbA family protein	-
IS499_RS27575	115325..115765	-	441	WP_011251275.1	hypothetical protein	-
IS499_RS27580	115755..116265	-	511	Protein_120	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..219405	219405
	-	inside	IScluster/Tn	-	-	93878..118185	24307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180652 WP_011251285.1 NZ_CP064236:c111276-110965 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180652 NZ_CP085622:c4194858-4194508 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT180652 WP_011251286.1 NZ_CP064236:c110968-110549 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180652 NZ_CP085622:c4195103-4194852 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1