Detailed information of TA system TA180631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 183684..184411
• Replicon: plasmid pJX5-1
• Accession: NZ_CP064233
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS503_RS26870
• Protein ID: WP_011251285.1
• Coordinates: 183684..183995 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS503_RS26875
• Protein ID: WP_011251286.1
• Coordinates: 183992..184411 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS503_RS26830	178745..179260	-	516	WP_041937820.1	hypothetical protein	-
IS503_RS26835	179368..179943	-	576	WP_074424927.1	hypothetical protein	-
IS503_RS26840	180060..181016	-	957	WP_011251280.1	DsbA family protein	-
IS503_RS26845	181076..181417	-	342	WP_011251281.1	hypothetical protein	-
IS503_RS26850	181431..181742	-	312	WP_011251282.1	hypothetical protein	-
IS503_RS26855	181759..182193	-	435	Protein_200	thioredoxin fold domain-containing protein	-
IS503_RS26860	182210..182907	-	698	Protein_201	IS1 family transposase	-
IS503_RS26865	183021..183479	+	459	Protein_202	DDE-type integrase/transposase/recombinase	-
IS503_RS26870	183684..183995	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS503_RS26875	183992..184411	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IS503_RS26880	184558..185526	+	969	WP_074428168.1	IS5 family transposase	-
IS503_RS26885	185628..185963	-	336	Protein_206	conjugal transfer protein TraI	-
IS503_RS26890	185977..186765	-	789	WP_040217257.1	hypothetical protein	-
IS503_RS26895	186786..187229	-	444	WP_048333569.1	hypothetical protein	-
IS503_RS26900	187825..188580	+	756	Protein_209	hypothetical protein	-
IS503_RS26905	188773..189243	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..228973	228973
	-	inside	IScluster/Tn	-	-	182210..194532	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180631 WP_011251285.1 NZ_CP064233:183684-183995 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180631 NZ_CP085620:c124292-123987 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT180631 WP_011251286.1 NZ_CP064233:183992-184411 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180631 NZ_CP085620:c124512-124294 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1