Detailed information of TA system TA180610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 197351..198078
• Replicon: plasmid pJX6-1
• Accession: NZ_CP064230
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS501_RS26890
• Protein ID: WP_011251285.1
• Coordinates: 197767..198078 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS501_RS26885
• Protein ID: WP_011251286.1
• Coordinates: 197351..197770 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS501_RS26855	192519..192989	-	471	WP_048333570.1	hypothetical protein	-
IS501_RS26860	193182..193937	-	756	Protein_202	hypothetical protein	-
IS501_RS26865	194533..194976	+	444	WP_048333569.1	hypothetical protein	-
IS501_RS26870	194997..195785	+	789	WP_040217257.1	hypothetical protein	-
IS501_RS26875	195799..196134	+	336	Protein_205	conjugal transfer protein TraI	-
IS501_RS26880	196236..197204	-	969	WP_074428168.1	IS5 family transposase	-
IS501_RS26885	197351..197770	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IS501_RS26890	197767..198078	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS501_RS26895	198283..198741	-	459	Protein_209	DDE-type integrase/transposase/recombinase	-
IS501_RS26900	198855..199552	+	698	Protein_210	IS1 family transposase	-
IS501_RS26905	199569..200003	+	435	Protein_211	thioredoxin fold domain-containing protein	-
IS501_RS26910	200020..200331	+	312	WP_011251282.1	hypothetical protein	-
IS501_RS26915	200345..200686	+	342	WP_011251281.1	hypothetical protein	-
IS501_RS26920	200746..201702	+	957	WP_011251280.1	DsbA family protein	-
IS501_RS26925	201819..202394	+	576	WP_074424927.1	hypothetical protein	-
IS501_RS26930	202502..203017	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228974	228974
	-	inside	IScluster/Tn	-	-	187230..199552	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180610 WP_011251285.1 NZ_CP064230:c198078-197767 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180610 NZ_CP085540:331-600 [Aeromonas salmonicida subsp. salmonicida]
TTGGCTTGGAAAGTTGAATTGACCGATACCGCCAAGAAGCAATTGGCTAGGCTGGATAAGACCCAATCGCAGAGGATCAC
AAAATATCTGCGGCGGATTATGATGCTTGAAAATCCGAGGGATGCTGGCAAAGCTCTAACCGGTAATTTACGAACCTACT
GGCGTTATCGCGTGGGGGATTATCGGGTTGTGTGTGACATAAGAGATAACGATCTTGTCATTGTTGCTGTGATAATTGGG
CATCGCAGTGAAGTATATGGTGGAAGTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT180610 WP_011251286.1 NZ_CP064230:c197770-197351 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180610 NZ_CP085540:123-347 [Aeromonas salmonicida subsp. salmonicida]
ATGTTAGCGATACGCTTGCCTTCGGAGATCGAAGAGCGTTTGGCAAATTTGGCGCAAGAGACGGGAAGAACGAAGACCTT
CTATGCACGGGAGGCCATCCTGACCCATCTGGAAGAGTTGGAAGATTACTATCTGGCTGAGAGGGTCATGGAGAATGTTC
GCAAGGGCGAAGAGCGCCTGTTCTCATTGGATGACGTGGAGCGCGATCTTGGCTTGGAAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1