Detailed information of TA system TA180587

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90688..90941
• Replicon: plasmid pJX7-3
• Accession: NZ_CP064226
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IS505_RS29380
• Protein ID: WP_001312851.1
• Coordinates: 90688..90837 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	90882..90941 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS505_RS29345	86047..86462	-	416	Protein_111	IS1 family transposase	-
IS505_RS29350	86711..87112	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IS505_RS29355	87045..87302	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IS505_RS29360	87395..88048	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IS505_RS29365	88987..89844	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IS505_RS29370	89837..89911	-	75	WP_001375168.1	RepA leader peptide Tap	-
IS505_RS29375	90156..90404	-	249	WP_000083837.1	replication regulatory protein RepA	-
IS505_RS29380	90688..90837	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	90882..90941	+	60	NuclAT_0	-	Antitoxin
-	90882..90941	+	60	NuclAT_0	-	Antitoxin
-	90882..90941	+	60	NuclAT_0	-	Antitoxin
-	90882..90941	+	60	NuclAT_0	-	Antitoxin
IS505_RS29385	91142..91372	-	231	WP_001736714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B	-	1..91913	91913
	-	inside	IScluster/Tn	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B	-	51777..86462	34685

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T180587 WP_001312851.1 NZ_CP064226:c90837-90688 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T180587 NZ_CP085532:c2365945-2365628 [Mycobacterium tuberculosis variant bovis BCG]
ATGACGCGTAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT180587 NZ_CP064226:90882-90941 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin