Detailed information of TA system TA180583

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67607..68033
• Replicon: plasmid pJX7-2
• Accession: NZ_CP064225
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS505_RS28430
• Protein ID: WP_001312861.1
• Coordinates: 67875..68033 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67607..67831 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS505_RS28405	63474..64001	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IS505_RS28410	64059..64292	+	234	WP_000006003.1	DUF905 family protein	-
IS505_RS28415	64353..66376	+	2024	Protein_82	ParB/RepB/Spo0J family partition protein	-
IS505_RS28420	66445..66879	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS505_RS28425	66876..67595	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	67607..67831	+	225	NuclAT_0	-	Antitoxin
-	67607..67831	+	225	NuclAT_0	-	Antitoxin
-	67607..67831	+	225	NuclAT_0	-	Antitoxin
-	67607..67831	+	225	NuclAT_0	-	Antitoxin
IS505_RS28430	67875..68033	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IS505_RS28435	68271..68648	-	378	Protein_86	hypothetical protein	-
IS505_RS28440	69198..69902	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS505_RS28445	69977..70252	+	276	Protein_88	PAS domain-containing protein	-
IS505_RS28450	70316..71020	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS505_RS28455	71054..71578	-	525	WP_094818827.1	recombinase family protein	-
IS505_RS28460	71971..72984	-	1014	WP_000845048.1	class 1 integron integrase IntI1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaLAP-2 / blaKPC-2 / dfrA14 / sul2	-	1..133575	133575

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180583 WP_001312861.1 NZ_CP064225:67875-68033 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180583 NZ_CP085532:c2205237-2204677 [Mycobacterium tuberculosis variant bovis BCG]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin

Nucleotide

• Length: 225 bp

>AT180583 NZ_CP064225:67607-67831 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin