Detailed information of TA system TA180580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 118883..119610
• Replicon: plasmid pJX7-1
• Accession: NZ_CP064224
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS505_RS27510
• Protein ID: WP_011251285.1
• Coordinates: 118883..119194 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS505_RS27515
• Protein ID: WP_011251286.1
• Coordinates: 119191..119610 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS505_RS27470	113894..114404	+	511	Protein_129	hypothetical protein	-
IS505_RS27475	114394..114834	+	441	WP_011251275.1	hypothetical protein	-
IS505_RS27480	115259..116215	-	957	WP_011251280.1	DsbA family protein	-
IS505_RS27485	116275..116616	-	342	WP_011251281.1	hypothetical protein	-
IS505_RS27490	116630..116941	-	312	WP_011251282.1	hypothetical protein	-
IS505_RS27495	116958..117392	-	435	Protein_134	thioredoxin fold domain-containing protein	-
IS505_RS27500	117409..118106	-	698	Protein_135	IS1 family transposase	-
IS505_RS27505	118220..118678	+	459	Protein_136	DDE-type integrase/transposase/recombinase	-
IS505_RS27510	118883..119194	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS505_RS27515	119191..119610	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IS505_RS27520	119757..120725	+	969	WP_074428168.1	IS5 family transposase	-
IS505_RS27525	120827..121162	-	336	Protein_140	conjugal transfer protein TraI	-
IS505_RS27530	121176..121964	-	789	WP_040217257.1	hypothetical protein	-
IS505_RS27535	121985..122428	-	444	WP_048333569.1	hypothetical protein	-
IS505_RS27540	123024..123779	+	756	Protein_143	hypothetical protein	-
IS505_RS27545	123972..124442	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN	1..219796	219796
	-	inside	IScluster/Tn	-	-	111974..141935	29961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180580 WP_011251285.1 NZ_CP064224:118883-119194 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180580 NZ_CP064224:118883-119194 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT180580 WP_011251286.1 NZ_CP064224:119191-119610 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180580 NZ_CP064224:119191-119610 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1