Detailed information of TA system TA180547

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44279..44705
• Replicon: plasmid pJX8-2
• Accession: NZ_CP064219
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-8

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS494_RS28130
• Protein ID: WP_001312861.1
• Coordinates: 44279..44437 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	44481..44705 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS494_RS28100	39676..40656	-	981	WP_013213985.1	IS481-like element ISKpn27 family transposase	-
IS494_RS28105	40779..41240	-	462	Protein_52	recombinase family protein	-
IS494_RS28110	41292..41996	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS494_RS28115	42060..42335	-	276	Protein_54	PAS domain-containing protein	-
IS494_RS28120	42410..43114	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS494_RS28125	43664..44041	+	378	Protein_56	hypothetical protein	-
IS494_RS28130	44279..44437	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	44481..44705	-	225	NuclAT_0	-	Antitoxin
-	44481..44705	-	225	NuclAT_0	-	Antitoxin
-	44481..44705	-	225	NuclAT_0	-	Antitoxin
-	44481..44705	-	225	NuclAT_0	-	Antitoxin
IS494_RS28135	44717..45436	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
IS494_RS28140	45433..45867	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS494_RS28145	45936..47959	-	2024	Protein_60	ParB/RepB/Spo0J family partition protein	-
IS494_RS28150	48020..48253	-	234	WP_000006003.1	DUF905 family protein	-
IS494_RS28155	48311..48838	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaLAP-2 / blaKPC-2 / blaSHV-12 / tet(A) / catA2 / dfrA14 / sul2	-	1..146760	146760

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180547 WP_001312861.1 NZ_CP064219:c44437-44279 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180547 NZ_CP085528:c1391249-1390953 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGGAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGACCGGGTTATCTCAAATACCCGGCAGGATCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGATGCCCAACGGCATTTGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT180547 NZ_CP064219:c44705-44481 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin