Detailed information of TA system TA180521

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 862..1115
• Replicon: plasmid pJX9-3
• Accession: NZ_CP064214
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IS495_RS28440
• Protein ID: WP_001312851.1
• Coordinates: 862..1011 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	1056..1115 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS495_RS28430	11..85	-	75	WP_001375168.1	RepA leader peptide Tap	-
IS495_RS28435	330..578	-	249	WP_000083837.1	replication regulatory protein RepA	-
IS495_RS28440	862..1011	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	1056..1115	+	60	NuclAT_0	-	Antitoxin
-	1056..1115	+	60	NuclAT_0	-	Antitoxin
-	1056..1115	+	60	NuclAT_0	-	Antitoxin
-	1056..1115	+	60	NuclAT_0	-	Antitoxin
IS495_RS28445	1316..1546	-	231	WP_001736714.1	hypothetical protein	-
IS495_RS28450	1710..2309	-	600	WP_032083981.1	hypothetical protein	-
IS495_RS28455	2695..2895	-	201	WP_015059022.1	hypothetical protein	-
IS495_RS28460	3027..3587	-	561	WP_000139328.1	fertility inhibition protein FinO	-
IS495_RS28465	3642..4388	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IS495_RS28470	4408..4569	-	162	Protein_9	DNA helicase	-
IS495_RS28475	4633..5337	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS495_RS28480	5389..5733	+	345	Protein_11	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B	-	1..90714	90714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T180521 WP_001312851.1 NZ_CP064214:c1011-862 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T180521 NZ_CP085506:2306609-2307346 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT180521 NZ_CP064214:1056-1115 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin