Detailed information of TA system TA180514

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 142819..143245
• Replicon: plasmid pJX9-1
• Accession: NZ_CP064212
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IS495_RS27040
• Protein ID: WP_001312861.1
• Coordinates: 142819..142977 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	143021..143245 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS495_RS27015	138423..138606	-	184	Protein_145	small membrane protein	-
IS495_RS27020	138626..139237	-	612	Protein_146	mucoid phenotype A regulator RmpA	-
IS495_RS27025	139369..140925	-	1557	WP_053897648.1	PAS domain-containing methyl-accepting chemotaxis protein	-
IS495_RS27030	140950..141654	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS495_RS27035	142204..142581	+	378	Protein_149	hypothetical protein	-
IS495_RS27040	142819..142977	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	143021..143245	-	225	NuclAT_0	-	Antitoxin
-	143021..143245	-	225	NuclAT_0	-	Antitoxin
-	143021..143245	-	225	NuclAT_0	-	Antitoxin
-	143021..143245	-	225	NuclAT_0	-	Antitoxin
IS495_RS27045	143257..143976	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
IS495_RS27050	143973..144407	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IS495_RS27055	144476..146499	-	2024	Protein_153	ParB/RepB/Spo0J family partition protein	-
IS495_RS27060	146560..146793	-	234	WP_000006003.1	DUF905 family protein	-
IS495_RS27065	146851..147378	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..277720	277720
	-	flank	IS/Tn	-	-	140950..141654	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180514 WP_001312861.1 NZ_CP064212:c142977-142819 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180514 NZ_CP085501:3015005-3015355 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT180514 NZ_CP064212:c143245-143021 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin